The Pharmacy School of Southwest Medical University, Luzhou, China; The Affiliated Hospital of Southwest Medical University, Luzhou, China.
The Affiliated Hospital of Traditional Chinese Medicine of Southwest Medical University, Luzhou, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Jan 5;224:117435. doi: 10.1016/j.saa.2019.117435. Epub 2019 Jul 29.
A novel two-photon pH probe, 3-benzimidazole-7-hydroxycoumarin (BHC), was designed and synthesized based on the structures of hydroxycoumarin and benzimidazole. BHC showed good linearity in the pH ranges of 3.30-5.40 (pKa = 4.20) and 6.50-8.30 (pKa = 7.20) at a maximum emission wavelength of 480 nm. BHC in acidic and alkaline media could be distinguished by an obvious spectral shift of the maximum absorption wavelength from 390 nm to 420 nm. In addition, BHC was well localized to mitochondria and successfully applied to one-photon and two-photon imaging of pH changes in the mitochondria of HeLa cells. The findings presented herein suggest that BHC can serve as an excellent fluorescent probe for selectively sensing mitochondrial pH changes with remarkable photostability and low cytotoxicity.
一种新型的双光子 pH 探针，3-苯并咪唑-7-羟基香豆素（BHC），是基于香豆素和苯并咪唑的结构设计和合成的。BHC 在最大发射波长为 480nm 时，在 pH 值为 3.30-5.40（pKa=4.20）和 6.50-8.30（pKa=7.20）的范围内显示出良好的线性关系。BHC 在酸性和碱性介质中可以通过最大吸收波长从 390nm 明显移动到 420nm 来区分。此外，BHC 很好地定位于线粒体，并成功地应用于 HeLa 细胞线粒体中 pH 变化的单光子和双光子成像。本文的研究结果表明，BHC 可以作为一种优异的荧光探针，用于选择性检测线粒体 pH 值的变化，具有显著的光稳定性和低细胞毒性。
