T-2毒素对小鼠迟发型超敏反应耐受诱导的抑制作用。

The inhibitory effect of T-2 toxin on tolerance induction of delayed-type hypersensitivity in mice.

作者信息

Otokawa M, Shibahara Y, Egashira Y

出版信息

Jpn J Med Sci Biol. 1979 Feb;32(1):37-45. doi: 10.7883/yoken1952.32.37.

DOI:10.7883/yoken1952.32.37

Abstract

Mice injected intravenously with 1 X 10(9) sheep red blood cells (SRBC) showed no delayed-type hypersensitivity (DTH) response to SRBC and were unresponsive to DTH induction by sc injection of an optimal dose of SRBC. However, when treated with T-2 toxin, a mycotoxin, 2 days after the iv injection, mice became to show significant DTH response and to be responsive to the DTH induction by the sc injection. When the spleen cells of the mice receiving the iv injection were transferred to unsensitized syngeneic recipients, the DTH response of the recipients to SRBC was suppressed. However, the suppressor activity of the spleen cells was decreased by T-2 toxin treatment. By the iv injection, cell population of the spleen was increased and that of the thymus decreased. In contrast, by T-2 toxin treatment 2 days after the iv injection, cell population of the spleen was not increased and that of the thymus was markedly decreased. The ratio of theta-bearing cells was increased in the spleen by the iv injection. However, such increase was not observed after the T-2 toxin treatment. The ratio of Ig-bearing cells in the spleen was not changed by the iv injection and the T-2 toxin treatment after the iv injection. T-2 toxin seems to interfere with generation of suppressor cells for the DTH response.

摘要

静脉注射1×10⁹个绵羊红细胞（SRBC）的小鼠对SRBC无迟发型超敏反应（DTH），且对皮下注射最佳剂量的SRBC诱导的DTH无反应。然而，在静脉注射后2天用真菌毒素T-2毒素处理，小鼠开始表现出显著的DTH反应，并对皮下注射诱导的DTH有反应。当将接受静脉注射的小鼠的脾细胞转移到未致敏的同基因受体时，受体对SRBC的DTH反应受到抑制。然而，T-2毒素处理降低了脾细胞的抑制活性。通过静脉注射，脾脏细胞数量增加，胸腺细胞数量减少。相反，在静脉注射后2天用T-2毒素处理，脾脏细胞数量没有增加，胸腺细胞数量明显减少。静脉注射使脾脏中θ阳性细胞的比例增加。然而，T-2毒素处理后未观察到这种增加。静脉注射以及静脉注射后T-2毒素处理均未改变脾脏中Ig阳性细胞的比例。T-2毒素似乎干扰了DTH反应抑制细胞的产生。