Hospices Civils de Lyon, Laboratoire de Virologie, Institut des Agents Infectieux, Centre de Biologie et de Pathologie Nord, 103, grande rue de la Croix-Rousse, 69 317, Lyon cedex 04, France.
Université Lyon 1 Claude Bernard, CIRI, Virpath, Inserm U1111-CNRS UMR 5308, ENS, Lyon, France.
Arch Virol. 2019 Nov;164(11):2775-2781. doi: 10.1007/s00705-019-04368-w. Epub 2019 Aug 10.
Diagnosis and epidemiological analysis of human parvovirus B19 (hB19V) infections are essential for disease management in severely ill patients. This study aimed to evaluate the performance of an optimized NS1-VP1u nested PCR for detection and sequencing of viruses in clinical samples using 224 clinical and five reference samples. PCR sensitivity, specificity, and positive and negative predictive values were perfect (100%). While phylogenetic analysis of a 615 bp-long fragment demonstrated that the viruses in all of the samples belonged to genotype 1, this study confirmed that this optimized PCR could detect all known hB19V with high performance.
诊断和流行病学分析人类细小病毒 B19（hB19V）感染对于重症患者的疾病管理至关重要。本研究旨在评估优化的 NS1-VP1u 巢式 PCR 在 224 份临床样本和 5 份参考样本中检测和测序病毒的性能。PCR 的敏感性、特异性、阳性和阴性预测值均为 100%。虽然对 615bp 长片段的系统发育分析表明，所有样本中的病毒均属于基因型 1，但本研究证实该优化的 PCR 能够以高的性能检测所有已知的 hB19V。
