Balise Victoria D, Cornelius-Green Jennifer N, Parmenter Brittany, Baxter Sierra, Kassotis Christopher D, Rector R Scott, Thyfault John P, Paterlini Silvia, Palanza Paola, Ruiz Daniel, Sargis Robert, Nagel Susan C
Department of Obstetrics, Gynecology and Women's Health, University of Missouri, Columbia, MO, United States.
Department of Biological Sciences, University of Missouri, Columbia, MO, United States.
Front Endocrinol (Lausanne). 2019 Jul 25;10:460. doi: 10.3389/fendo.2019.00460. eCollection 2019.
Chemicals used in unconventional oil and gas (UOG) operations can act as endocrine disrupting chemicals and metabolic disruptors. Our lab has reported altered energy expenditure and activity in C57BL/6J mice that were preconceptionally, gestationally, and lactationally exposed via maternal drinking water to a laboratory-created mixture of 23 UOG chemicals from gestational day 1 to postnatal day 21 in 7-month-old female mice with no change in body composition. We hypothesized that allowing the mice to age and exposing them to a high fat, high sugar diet might reveal underlying changes in energy balance. To investigate whether aging and metabolic challenge would exacerbate this phenotype, these mice were aged to 12 months and given a high fat, high sugar diet (HFHSD) challenge. The short 3-day HFHSD challenge increased body weight and fasting blood glucose in all mice. Developmental exposure to the 23 UOG mixture was associated with increased activity and non-resting energy expenditure in the light cycle, increased exploratory behavior in the elevated plus maze test, and decreased sleep in 12 month female mice. Each of these effects was seen in the light cycle when mice are normally less active. Further studies are needed to better understand the behavioral changes observed after developmental exposure to UOG chemicals.
非常规石油和天然气（UOG）作业中使用的化学物质可作为内分泌干扰物和代谢干扰物。我们实验室报告称，C57BL/6J小鼠在受孕前、妊娠期和哺乳期通过母体饮用水，从妊娠第1天到出生后第21天接触实验室配制的23种UOG化学物质混合物，在7月龄雌性小鼠中能量消耗和活动发生了改变，而身体组成没有变化。我们推测，让小鼠变老并使其接触高脂肪、高糖饮食可能会揭示能量平衡的潜在变化。为了研究衰老和代谢挑战是否会加剧这种表型，将这些小鼠饲养到12个月龄，并给予高脂肪、高糖饮食（HFHSD）挑战。为期3天的短期HFHSD挑战使所有小鼠的体重和空腹血糖增加。发育过程中接触23种UOG混合物与12月龄雌性小鼠在光照周期中活动增加和非静息能量消耗增加、高架十字迷宫试验中探索行为增加以及睡眠减少有关。这些影响在小鼠通常活动较少的光照周期中均有出现。需要进一步研究以更好地理解发育过程中接触UOG化学物质后观察到的行为变化。
