Li Guofeng
Department of Anorectal Surgery, The First People's Hospital of Xiaoshan Hangzhou, Hangzhou, Zhejiang 311225, P.R. China.
Oncol Lett. 2019 Sep;18(3):2278-2285. doi: 10.3892/ol.2019.10566. Epub 2019 Jul 5.
Expression of gene and miR-363 in colorectal cancer was studied to explore its relationship with clinicopathological characteristics of colorectal cancer and to analyze the value of combined with miR-363 in the diagnosis of colorectal cancer. In total, 85 patients diagnosed with colorectal cancer in the First Peoples Hospital of Xiaoshan Hangzhou from March 2014 to July 2016 were the experiment group. Seventy healthy individuals who underwent physical examination were the control group. RT-qPCR was used to detect the expression levels of gene and miR-363 in peripheral blood of the two groups. The relationship between the expression of and miR-363 with its clinicopathological characteristics was analyzed as well. The expression of in the experiment group was significantly lower than that in the control group (P<0.05). The expression level of miR-363 was significantly lower than in the control group (P<0.05). However, there was a correlation with tumor size, degree of differentiation, lymph node metastasis, depth of invasion and clinical stages (P<0.05). and miR-363 were significantly positively correlated with the degree of differentiation (r=0.7381, r=0.5375; P<0.05); and miR-363 were significantly negatively correlated with clinical stages (r=-0.7167, -0.6700; P<0.05). The area under the ROC curve of the combined test was larger than the single test. The expression of gene and miR-363 in peripheral blood of patients with colorectal cancer was lower than in the normal controls. The low expression of and miR-363 was closely related to various biological behaviors of colorectal cancer. A potential reference is provided for the evaluation of patients with colorectal cancer and expected to have an important guiding effect in the treatment of colorectal cancer. Moreover, combined test of and miR-363 has important significance in the diagnosis and treatment evaluation of colorectal cancer.
研究了基因和miR - 363在结直肠癌中的表达，以探讨其与结直肠癌临床病理特征的关系，并分析其与miR - 363联合在结直肠癌诊断中的价值。2014年3月至2016年7月在杭州市萧山区第一人民医院确诊为结直肠癌的85例患者作为实验组。70例接受体检的健康个体作为对照组。采用RT - qPCR检测两组外周血中基因和miR - 363的表达水平。同时分析了与miR - 363的表达与其临床病理特征之间的关系。实验组中基因的表达明显低于对照组（P<0.05）。miR - 363的表达水平明显低于对照组（P<0.05）。然而，其与肿瘤大小、分化程度、淋巴结转移、浸润深度及临床分期存在相关性（P<0.05）。与miR - 363与分化程度呈显著正相关（r = 0.7381，r = 0.5375；P<0.05）；与miR - 363与临床分期呈显著负相关（r = - 0.7167， - 0.6700；P<0.05）。联合检测的ROC曲线下面积大于单项检测。结直肠癌患者外周血中基因和miR - 363的表达低于正常对照组。基因和miR - 363的低表达与结直肠癌的各种生物学行为密切相关。为结直肠癌患者的评估提供了潜在参考，有望在结直肠癌的治疗中发挥重要指导作用。此外，与miR - 363联合检测在结直肠癌的诊断和治疗评估中具有重要意义。
