Fan Zhenwei, Wang Xuan, Li Peng, Mei Chunli, Zhang Min, Zhao Chunshan
Nursing College of Beihua University, Jilin City, Jilin 132013, P.R. China.
Department of Hematology, Affiliated Hospital of Beihua University, Jilin City, Jilin 132013, P.R. China.
Oncol Lett. 2019 Sep;18(3):2443-2447. doi: 10.3892/ol.2019.10540. Epub 2019 Jun 28.
Long non-coding RNA GATA6 antisense RNA 1 (lncRNA GATA6-AS) is a recently identified lncRNA that is involved in endothelial-mesenchymal transition. The present study aimed to investigate the involvement of GATA6-AS in the progression of mantle cell lymphoma (MCL). It was found that plasma lncRNA GATA6-AS expression level was downregulated in patients with MCL, compared with that in healthy controls. Downregulation of lncRNA GATA6-AS has potential diagnostic value in early stage MCL. Overexpression of lncRNA GATA6-AS resulted in inhibited glucose uptake in the human cell lines JVM-2 and Z-138 MCL. Inhibited expression of glucose transporter 1 (GLUT1) was observed in MCL cells following lncRNA GATA6-AS overexpression, whilst GLUT1 overexpression did not alter the expression of lncRNA GATA6-AS. Additionally, lncRNA GATA6-AS overexpression inhibited, whilst GLUT1 overexpression promoted the proliferation of JVM-2 and Z-138 MCL cells; GLUT1 overexpression partially reversed the inhibitory effects of lncRNA GATA6-AS overexpression. It was therefore concluded that lncRNA GATA6-AS may inhibit cancer cell proliferation in MCL by downregulating GLUT1.
长链非编码RNA GATA6反义RNA 1（lncRNA GATA6-AS）是一种最近被鉴定出的参与内皮-间充质转化的lncRNA。本研究旨在探讨GATA6-AS在套细胞淋巴瘤（MCL）进展中的作用。研究发现，与健康对照相比，MCL患者血浆lncRNA GATA6-AS表达水平下调。lncRNA GATA6-AS的下调在早期MCL中具有潜在的诊断价值。lncRNA GATA6-AS的过表达导致人MCL细胞系JVM-2和Z-138的葡萄糖摄取受到抑制。lncRNA GATA6-AS过表达后，MCL细胞中葡萄糖转运蛋白1（GLUT1）的表达受到抑制，而GLUT1的过表达并未改变lncRNA GATA6-AS的表达。此外，lncRNA GATA6-AS的过表达抑制了JVM-2和Z-138 MCL细胞的增殖，而GLUT1的过表达则促进了这些细胞的增殖；GLUT1的过表达部分逆转了lncRNA GATA6-AS过表达的抑制作用。因此得出结论，lncRNA GATA6-AS可能通过下调GLUT1来抑制MCL中的癌细胞增殖。
