Maeda Paula M, Bicudo Ana Paula S L, Watanabe Renata T M, Fonseca Thais S M, do Souto Ricardo P, Fernandes César E, de Oliveira Emerson
Department of Urogynecology and Vaginal Surgery of the Discipline of Gynecology, ABC Medical School, Santo André, SP, Brazil.
Amaro Soares França 301 St., Alto do Córrego, Paracatu, MG, 38600-000, Brazil.
Eur J Obstet Gynecol Reprod Biol X. 2019 Apr 30;3:100031. doi: 10.1016/j.eurox.2019.100031. eCollection 2019 Jul.
To analyze the polymorphism -1171 5A / 6A rs3025058 of the MMP-3 gene and the risk for pelvic organ prolapse (POP).
This is a cohort study. All patients attended the Urogynecology and Vaginal Surgery Section of the FMABC, from 2014 to 2016 and they were randomly recruited by the researchers at the first medical appointment. We selected 112 patients with symptomatic POPs and 180 patients with normal pelvic floors. The single nucleotide polymorphism (SNP) 5A / 6A of MMP-3 was determined by polymerase chain reaction (PCR) and analysis of the restriction fragments in both groups. Chi-squared test was used to compare the frequencies of polymorphisms between the groups. For those characteristics with statistical relevance, the crude odds ratio (OR) and its respective 95% confidence intervals were calculated; and, by logistic regression, were adjusted for each of the other characteristics, obtaining the adjusted OR. Hardy-Weinberg gene balance was determined using Pearson's Chi-squared test. Values of p < 0.05 were considered statistically significant.
Logistic regression of factors associated with genital prolapse showed that age (adjusted OR = 11.89, 95% CI, 3.53-40) and home delivery (adjusted OR = 9.645, 95% CI, 3.35-27.7) remained risk factors for genital prolapse in the sample studied. There was no statistically significant difference between the groups in the distribution of genotypes, even after calculating the contribution of the 5A recessive allele in the aggregated genotypes (5A / 5A + 5A / 6A).
The polymorphism -1171 5A / 6A rs3025058 of the MMP-3 gene was not associated with the risk for POP. Age and home delivery were significantly associated with increased risk for the disease.
分析基质金属蛋白酶-3（MMP-3）基因-1171 5A/6A rs3025058多态性与盆腔器官脱垂（POP）风险的关系。
这是一项队列研究。2014年至2016年期间，所有患者均就诊于FMABC的泌尿妇科和阴道外科，研究人员在首次就诊时随机招募患者。我们选取了112例有症状的盆腔器官脱垂患者和180例盆底正常的患者。通过聚合酶链反应（PCR）及对两组限制性片段的分析来确定MMP-3的单核苷酸多态性（SNP）5A/6A。采用卡方检验比较两组间多态性频率。对于具有统计学相关性的特征，计算粗比值比（OR）及其相应的95%置信区间；并通过逻辑回归对其他每个特征进行校正，得出校正后的OR。使用Pearson卡方检验确定Hardy-Weinberg基因平衡。p<0.05的值被认为具有统计学意义。
与生殖器脱垂相关因素的逻辑回归显示，在所研究的样本中，年龄（校正后的OR = 11.89，95% CI，3.53 - 40）和在家分娩（校正后的OR = 9.645，95% CI，3.35 - 27.7）仍然是生殖器脱垂的危险因素。即使在计算聚集基因型（5A/5A + 5A/6A）中5A隐性等位基因的贡献后，两组间基因型分布仍无统计学显著差异。
MMP-3基因-1171 5A/6A rs3025058多态性与盆腔器官脱垂风险无关。年龄和在家分娩与该疾病风险增加显著相关。
