Saw Chiou-Yeong, Chang Tsai-Ju, Chen Po-Ya, Dai Fan-Jhen, Lau Yie-Qie, Chen Ting-Yu, Chau Chi-Fai
Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan.
Biosci Biotechnol Biochem. 2019 Dec;83(12):2327-2333. doi: 10.1080/09168451.2019.1651628. Epub 2019 Aug 12.
This study was aimed to investigate the presence of vegetative cells in the intestine and fecal samples in rats fed spores as well as to estimate the ratios of spores and vegetative cells in these samples. A two-step process has been developed to enumerate in different mixed bacterial samples, specifically (1) observation of yellow ring formation on modified GYEA medium upon incubation at 55°C, (2) microscopic examination of spore formation after 7 d of incubation. Our results have demonstrated the presence of vegetative cells in the intestinal and fecal samples in rats fed spores. The ratios of spores and vegetative cells in cecal fluid, colonic content, and feces were approximately 2:8, 2:8, and 4:6, respectively. The existence of vegetative cells improved the intestinal milieu through an elevated short-chain fatty acid concentrations, higher fecal moisture, and lower fecal pH.
本研究旨在调查喂食孢子的大鼠肠道和粪便样本中营养细胞的存在情况，并估计这些样本中孢子与营养细胞的比例。已开发出一种两步法来对不同的混合细菌样本进行计数，具体如下：（1）在55°C孵育后观察改良GYEA培养基上黄色环的形成；（2）孵育7天后对孢子形成进行显微镜检查。我们的结果表明，喂食孢子的大鼠肠道和粪便样本中存在营养细胞。盲肠液、结肠内容物和粪便中孢子与营养细胞的比例分别约为2:8、2:8和4:6。营养细胞的存在通过提高短链脂肪酸浓度、增加粪便水分和降低粪便pH值改善了肠道环境。
