Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
PLoS One. 2019 Aug 12;14(8):e0218379. doi: 10.1371/journal.pone.0218379. eCollection 2019.
A multi-trait selective breeding program of Macrobrachium rosenbergii was initiated in China in 2015. In this program, the M. rosenbergii resources were widely collected from four countries, the origin of the founders was verified with 16 microsatellites and the pedigree was reconstructed, and the optimum contribution selection was used to make the mating design. In this study, we evaluated the genetic parameters and selection response for the harvest body weight (HBW) of M. rosenbergii after being communally reared for 95-109 days. The data were collected from two generations that comprised 25,212 progenies from 150 sires and 198 dams. The residual maximum-likelihood methodology was employed to evaluate the variance components, by fitting an animal model. The accuracy of estimated breeding values increased by 0.38% after pedigree reconstruction using microsatellite markers. The estimated heritability (h2) for HBW was moderate (0.212 ± 0.049) and the common environmental coefficient (c2) was low (0.063 ± 0.017) when all the data were used for the analysis. Within generations, h2 was moderate to high (0.198 ± 0.080 to 0.338 ± 0.049). c2 could only be estimated in G1, which was 0.055 ± 0.030. The average HBW of males was significantly larger than that of females (P < 0.01). h2 estimated for female HBWs were higher than that for males within generations, while h2 estimated for female HBWs were lower than that for males across generations. But they were not significantly different (P > 0.05). The genetic correlations between sexes were moderate to high within each generation (0.529 to 0.763). Two methods were used to estimate the realized response. One method was calculated from the differences between the least squares means of the selected population HBW and that of control population HBW, which was 14.01%. The other method was calculated from the differences between the EBVs of the selected population HBW and that of control population HBW, which was 11.52%. The predicted responses derived from two sets of genetic parameters acquired from within- and across- generation datasets were 11.68% and 10.67%, respectively. The present study provides valuable information for breeding programs of M. rosenbergii.
2015 年，中国启动了罗氏沼虾的多性状选择性育种计划。在该计划中，从四个国家广泛收集了罗氏沼虾资源，利用 16 个微卫星对其起源进行了验证，并重建了系谱，同时采用最佳贡献选择进行了交配设计。本研究评估了罗氏沼虾共同养殖 95-109 天后收获体重（HBW）的遗传参数和选择响应。该数据来自两代，共 150 个父本和 198 个母本的 25212 个子代。利用剩余最大似然法，通过拟合动物模型，评估方差分量。利用微卫星标记进行系谱重建后，估计育种值的准确性提高了 0.38%。HBW 的估计遗传力（h2）适中（0.212 ± 0.049），共同环境系数（c2）较低（0.063 ± 0.017），当所有数据都用于分析时。在各世代内，h2 为中等至高（0.198 ± 0.080 至 0.338 ± 0.049）。仅在 G1 中可以估计 c2，为 0.055 ± 0.030。雄性的平均 HBW 显著大于雌性（P < 0.01）。各世代内，雌性 HBW 的 h2 估计值高于雄性，而跨世代时，雌性 HBW 的 h2 估计值低于雄性，但差异不显著（P > 0.05）。同一世代内，雌雄之间的遗传相关性为中等至高（0.529 至 0.763）。使用两种方法来估计实际响应。一种方法是从选择种群 HBW 的最小二乘均值与对照种群 HBW 的最小二乘均值之差计算，为 14.01%。另一种方法是从选择种群 HBW 的 EBV 与对照种群 HBW 的 EBV 之差计算，为 11.52%。从跨世代和世代内数据集获得的两组遗传参数预测的响应分别为 11.68%和 10.67%。本研究为罗氏沼虾的育种计划提供了有价值的信息。
