Department of Bioengineering, Izmir Institute of Technology (IzTech), 35430 Izmir, Turkey.
Department of Chemistry, Izmir Institute of Technology (IzTech), 35430 Izmir, Turkey.
Int J Biol Macromol. 2019 Oct 15;139:1054-1062. doi: 10.1016/j.ijbiomac.2019.08.082. Epub 2019 Aug 9.
Electrospun collagen is commonly used as a scaffold in tissue engineering applications since it mimics the content and morphology of native extracellular matrix (ECM) well. This report describes "toxic solvent free" fabrication of electrospun hybrid scaffold consisting of Collagen (Col) and Poly(l-lactide-co-ε-caprolactone) (PLLCL) for three-dimensional (3D) cell culture. Biomimetic hybrid scaffold was fabricated via co-spinning approach where simultaneous electrospinning of PLLCL and Collagen was mediated by polymer sacrificing agent Polyvinylpyrrolidone (PVP). Acidified aqueous solution of PVP was used to solubilize collagen without using toxic solvents for electrospinning, and then PVP was readily removed by rinsing in water. Mechanical characterizations, protein adsorption, as well as biodegradation analysis have been conducted to investigate feasibility of biomimetic hybrid scaffold for 3D cell culture applications. Electrospun biomimetic hybrid scaffold, which has 3D-network structure with 300-450 nm fiber diameters, was found to be maximizing cell adhesion through assisting NIH 3T3 mouse fibroblast cells. 3D cell culture studies confirmed that presence of collagen in biomimetic hybrid scaffold have created a major impact on cell proliferation compared to conventional 2D systems on long-term, also cell viability increased with the increasing amount of collagen.
静电纺丝胶原通常被用作组织工程应用中的支架，因为它很好地模拟了天然细胞外基质（ECM）的含量和形态。本报告描述了由胶原（Col）和聚（L-丙交酯-共-ε-己内酯）（PLLCL）组成的“无毒性溶剂”电纺混合支架的制造，用于三维（3D）细胞培养。仿生混合支架是通过共纺方法制造的，其中 PLLCL 和胶原的同时电纺是通过聚合物牺牲剂聚乙烯吡咯烷酮（PVP）介导的。使用酸性 PVP 水溶液溶解胶原，而无需使用有毒溶剂进行电纺，然后通过在水中冲洗即可轻松去除 PVP。已经进行了机械特性、蛋白质吸附以及生物降解分析，以研究仿生混合支架用于 3D 细胞培养应用的可行性。电纺仿生混合支架具有 300-450nm 纤维直径的 3D 网络结构，被发现通过辅助 NIH 3T3 小鼠成纤维细胞最大限度地促进细胞黏附。3D 细胞培养研究证实，与传统的 2D 系统相比，仿生混合支架中胶原的存在对细胞增殖产生了重大影响，而且随着胶原含量的增加，细胞活力也增加了。
