Tang Chuanqing, Lu Chunmei, Ji Xiaoli, Ma Ling, Zhou Qi, Xiong Man, Zhou Wenhao
Stem Cell Research Center, Institute of Pediatrics, Children's Hospital, Fudan University, 399 Wanyuan Road, Shanghai 201102, China.
Department of Neonatology, Children's Hospital of Fudan University, Shanghai, China.
Stem Cell Res. 2019 Aug;39:101511. doi: 10.1016/j.scr.2019.101511. Epub 2019 Jul 27.
Human breast milk epithelial cells (BMECs) can be isolated and cultured with high purity. Induced pluripotent stem cells (iPSCs) were generated from BMECs with Yamanaka factors (OCT4, SOX2, c-MYC, KLF4) using episomal system. Pluripotency of breast milk-derived iPSCs (BM-iPSCs) was confirmed by the expression of pluripotent markers with immunocytochemistry and spontaneous differentiation of three germ layers in vitro and teratoma formation assay in vivo. Besides, the iPSC lines displayed normal karyotype. Breast milk is a non-invasive and easily accessible cell source, we can obtain BM-iPSCs from BMECs with low costs in a transgene-free episomal system.
人母乳上皮细胞（BMECs）能够以高纯度进行分离和培养。利用游离型载体系统，通过山中因子（OCT4、SOX2、c-MYC、KLF4）从BMECs中诱导生成诱导多能干细胞（iPSCs）。通过免疫细胞化学检测多能性标志物的表达、体外三个胚层的自发分化以及体内畸胎瘤形成试验，证实了母乳来源的iPSCs（BM-iPSCs）的多能性。此外，iPSC系显示出正常的核型。母乳是一种非侵入性且易于获取的细胞来源，我们可以在无转基因的游离型载体系统中以低成本从BMECs获得BM-iPSCs。
