School of Anesthesiology, Xuzhou Medical University, Xuzhou 221000, Jiangsu, China.
Department of Nephrology, Xuzhou Municipal Hospital Affiliated to Xuzhou Medical University, Xuzhou 221000, Jiangsu, China.
Biomed Pharmacother. 2019 Oct;118:109307. doi: 10.1016/j.biopha.2019.109307. Epub 2019 Aug 9.
Sepsis is a severe, life-threatening condition caused primarily by the cellular response to infection. Sepsis leads to increased tissue damage and mortality in patients in the intensive care unit. L-Lysine is an essential amino acid required for protein biosynthesis and is abundant in lamb, pork, eggs, red meat, fish oil, cheese, beans, peas, and soy. The present study investigates the protective effect of L-lysine against sepsis-induced acute lung injury (ALI) in a lipopolysaccharide-induced mouse model. In the present study, mice were divided into sham, control, 5 mg/kg body weight L-lysine, and 10 mg/kg body weight L-lysine treatment groups. At the end of the treatment period, we determined the levels of oxidative and inflammatory markers, myeloperoxidase (MPO) and catalase activities, total cell count, the wet/dry ratio of lung tissue, and total protein content. The effects of L-lysine on the cellular architecture of lung tissue were also evaluated. L-Lysine treatment significantly reduced the magnitude of lipid peroxidation; total protein content; wet/dry ratio of lung tissue; tumor necrosis factor alpha, interleukin-8, and macrophage inhibitory factor levels; MPO activity; and total cell, neutrophil, and lymphocyte counts. It also increased the levels of reduced glutathione and the activities of glutathione peroxidase, superoxide dismutase, and catalase. A normal cellular architecture was noted in mice in the sham group, whereas proinflammatory changes such as edema and neutrophilic infiltration were detected in mice in the control group. L-lysine significantly ameliorated these proinflammatory changes. Taking all these data together, it is suggested that the L-lysine was effective against sepsis-induced ALI.
脓毒症是一种严重的、危及生命的疾病，主要由细胞对感染的反应引起。脓毒症导致重症监护病房患者的组织损伤和死亡率增加。L-赖氨酸是蛋白质生物合成所需的必需氨基酸，在羊肉、猪肉、鸡蛋、红肉、鱼油、奶酪、豆类、豌豆和大豆中含量丰富。本研究探讨了 L-赖氨酸对脂多糖诱导的小鼠模型中脓毒症诱导的急性肺损伤（ALI）的保护作用。在本研究中，将小鼠分为假手术组、对照组、5mg/kg 体重 L-赖氨酸组和 10mg/kg 体重 L-赖氨酸组。在治疗期末，我们测定了氧化和炎症标志物、髓过氧化物酶（MPO）和过氧化氢酶活性、总细胞计数、肺组织湿/干比和总蛋白含量的水平。还评估了 L-赖氨酸对肺组织细胞结构的影响。L-赖氨酸治疗显著降低了脂质过氧化、总蛋白含量、肺组织湿/干比、肿瘤坏死因子-α、白细胞介素-8 和巨噬细胞抑制因子水平、MPO 活性以及总细胞、中性粒细胞和淋巴细胞计数的幅度。它还增加了还原型谷胱甘肽的水平以及谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶的活性。假手术组的小鼠肺组织呈现正常的细胞结构，而对照组的小鼠则出现了促炎变化，如水肿和中性粒细胞浸润。L-赖氨酸显著改善了这些促炎变化。综合所有这些数据表明，L-赖氨酸对脓毒症诱导的 ALI 有效。
