Jang Chul Ho, Park H, Cho Y B, Choi C H
Department of Otolaryngology, Chonnam National University Medical School, Dongku, South Korea.
J Laryngol Otol. 2010 Jun;124(6):594-8. doi: 10.1017/S0022215109992672. Epub 2010 Jan 8.
Bacterial biofilm formation has been implicated in the high incidence of persistent otorrhoea after tympanostomy tube insertion. It has been suggested that the tube material may be an important factor in the persistence of such otorrhoea. Development of methicillin-resistant Staphylococcus aureus otorrhoea after tympanostomy tube placement is a growing concern. We evaluated the effect of using vancomycin and chitosan coated tympanostomy tubes on the incidence of methicillin-resistant Staphylococcus aureus biofilm formation in vitro.
Three sets each of vancomycin-coated silicone tubes (n = 5), commercial silver oxide coated silicone tubes (n = 5) and uncoated tympanostomy tubes (as controls; n = 5) were compared as regards resistance to methicillin-resistant Staphylococcus aureus biofilm formation after in vitro incubation.
Scanning electron microscopy showed that the surfaces of the silver oxide coated tubes supported the formation of thick biofilms with crusts, comparable to the appearance of the uncoated tubes. In contrast, the surface of the vancomycin-coated tympanostomy tubes was virtually devoid of methicillin-resistant Staphylococcus aureus biofilm.
Vancomycin-coated tympanostomy tubes resist methicillin-resistant Staphylococcus aureus biofilm formation. Pending further study, such tubes show promise in assisting the control of methicillin-resistant Staphylococcus aureus biofilm formation.
细菌生物膜形成与鼓膜置管术后持续性耳漏的高发生率有关。有人提出，置管材料可能是此类耳漏持续存在的一个重要因素。鼓膜置管后耐甲氧西林金黄色葡萄球菌耳漏的发生日益受到关注。我们评估了使用万古霉素和壳聚糖涂层鼓膜置管对体外耐甲氧西林金黄色葡萄球菌生物膜形成发生率的影响。
比较三组鼓膜置管，每组各5根，分别为万古霉素涂层硅胶管、市售氧化银涂层硅胶管和未涂层鼓膜置管（作为对照），比较体外培养后对耐甲氧西林金黄色葡萄球菌生物膜形成的抗性。
扫描电子显微镜显示，氧化银涂层管的表面支持形成有结痂的厚生物膜，与未涂层管的外观相当。相比之下，万古霉素涂层鼓膜置管的表面几乎没有耐甲氧西林金黄色葡萄球菌生物膜。
万古霉素涂层鼓膜置管可抵抗耐甲氧西林金黄色葡萄球菌生物膜的形成。在进一步研究之前，此类置管在协助控制耐甲氧西林金黄色葡萄球菌生物膜形成方面显示出前景。
