Paul L T, Atilan O, Tulay P
Near East University, Institute of Health Sciences, Department of Medical Biology and Genetics, Nicosia, Cyprus.
Near East University, Faculty of Medicine, Department of Medical Genetics, Nicosia, Cyprus.
Zygote. 2019 Oct;27(5):347-349. doi: 10.1017/S0967199419000418. Epub 2019 Aug 13.
The aim of this study was to investigate if there is an adverse effect of multiple controlled ovarian stimulation (COS) on the maturity of oocytes (MI and MII), fertilization rate, embryo developmental qualities and clinical pregnancy rates in donation cycles. In total, 65 patients undergoing oocyte donation cycles multiple times were included in this study. Patients were grouped as group A that consisted of donors with ≤2 stimulation cycles while B consisted of donors with ≥3 stimulation cycles; and group C included donors who had ≤15 oocytes, while group D had donors with ≥16 oocytes. Numbers of oocytes obtained, MI and MII oocytes, fertilization, embryo quality and clinical pregnancy outcomes were compared. Significant statistical differences were observed in total number of oocytes obtained, maturity of oocytes (MI and MII), fertilization rate, embryo qualities and clinical pregnancy outcomes of donors in groups A-D. Donors with ≤2 ovarian stimulation cycles had lower numbers of immature oocytes than donors with three or more stimulation cycles. However, donors with ≥3 stimulation cycles had higher numbers of mature oocytes, zygotes, with better day 3 embryo qualities and higher clinical pregnancy rates than donors with ≤2 stimulation cycles. Repeated COS does not seem to have any adverse effect on ovarian response to higher dose of artificial gonadotropin, as quality of oocytes collected and their embryological developmental potential were not affected by the number of successive stimulation cycles. The effect of multiple COS on the health of the oocyte donor needs to be assessed for future purpose.
本研究的目的是调查多次控制性卵巢刺激（COS）对供卵周期中卵母细胞成熟度（MI和MII）、受精率、胚胎发育质量及临床妊娠率是否存在不利影响。本研究共纳入65例多次接受卵母细胞捐赠周期的患者。患者分为A组，该组由刺激周期≤2次的供者组成，B组由刺激周期≥3次的供者组成；C组包括卵母细胞≤15个的供者，D组包括卵母细胞≥16个的供者。比较获取的卵母细胞数量、MI和MII期卵母细胞、受精情况、胚胎质量及临床妊娠结局。在A - D组供者获取的卵母细胞总数、卵母细胞成熟度（MI和MII）、受精率、胚胎质量及临床妊娠结局方面观察到显著的统计学差异。刺激周期≤2次的供者未成熟卵母细胞数量低于刺激周期为三次或更多次的供者。然而，刺激周期≥3次的供者成熟卵母细胞、受精卵数量更多，第3天胚胎质量更好，临床妊娠率高于刺激周期≤2次的供者。重复COS似乎对卵巢对更高剂量人工促性腺激素的反应没有任何不利影响，因为采集的卵母细胞质量及其胚胎发育潜能不受连续刺激周期次数的影响。为了未来的目的，需要评估多次COS对卵母细胞供者健康的影响。
