Nepomuceno Rafael, Vallerini Bruna de F, da Silva Romerito L, Corbi Sâmia C T, Bastos Alliny de S, Dos Santos Raquel A, Takahashi Catarina S, Orrico Silvana Regina P, Scarel-Caminaga Raquel M
Department of Morphology, School of Dentistry at Araraquara, UNESP - São Paulo State University, Araraquara - SP, 14801-903, Brazil; Department of Diagnosis and Surgery, School of Dentistry at Araraquara, UNESP - São Paulo State University, Araraquara - SP, 14801-903, Brazil.
Department of Morphology, School of Dentistry at Araraquara, UNESP - São Paulo State University, Araraquara - SP, 14801-903, Brazil.
Diabetes Metab Syndr. 2019 Jul-Aug;13(4):2715-2722. doi: 10.1016/j.dsx.2019.07.003. Epub 2019 Jul 8.
Inflammatory diseases, as periodontal disease (PD), has been associated with disturbance of lipid and glycemic metabolisms, as demonstrated by the increasing of PD patients with type 2 diabetes mellitus (T2D) and/or dyslipidemia comorbidities. We aimed to investigate the expression of inflammation and lipid metabolism genes, and correlations among clinical and biochemical characteristics in normoglycemic or T2D patients with dyslipidemia and PD, in comparison with healthy individuals. Five groups of 30 individuals each (150 patients) were formed based upon T2D, dyslipidemic and periodontal status. Blood analyses of lipid and glycemic profiles were carried out, and the gene expression was assessed by RT-qPCR. The systemic expression of IL6, TNFA and LEP genes were significantly higher in T2D, dyslipidemia and PD patients, while the PECAM1 gene showed the opposite. Higher RETN levels were found in patients with T2D independently of their glycemic control status. There were positive correlations between: TNFA, LEP and RETN with worse periodontal parameters; IL6, TNFA, ADIPOR1, LEP and RETN with waist-to-hip ratio; glycemic parameters with RETN; total cholesterol and triglycerides with LEP expression. We conclude that pro-inflammatory cytokines were related with worse lipid, glycemic and periodontal parameters, reinforcing that a hyper-inflammatory status connects systemic and oral inflammatory diseases.
炎症性疾病，如牙周病（PD），已被证明与脂质和血糖代谢紊乱有关，2型糖尿病（T2D）和/或血脂异常合并症的PD患者数量增加就证明了这一点。我们旨在研究血糖正常或患有血脂异常和PD的T2D患者中炎症和脂质代谢基因的表达，以及临床和生化特征之间的相关性，并与健康个体进行比较。根据T2D、血脂异常和牙周状况将30人分为五组（共150名患者）。进行了血脂和血糖谱的血液分析，并通过RT-qPCR评估基因表达。T2D、血脂异常和PD患者中IL6、TNFA和LEP基因的全身表达显著更高，而PECAM1基因则相反。在T2D患者中发现RETN水平较高，与他们的血糖控制状态无关。TNFA、LEP和RETN与较差的牙周参数之间存在正相关；IL6、TNFA、ADIPOR1、LEP和RETN与腰臀比之间存在正相关；血糖参数与RETN之间存在正相关；总胆固醇和甘油三酯与LEP表达之间存在正相关。我们得出结论，促炎细胞因子与较差的脂质、血糖和牙周参数有关，这进一步证明了高炎症状态将全身性和口腔炎症性疾病联系在一起。
