Nepomuceno Rafael, Villela Bárbara Scoralick, Corbi Sâmia Cruz Tfaile, Bastos Alliny De Souza, Dos Santos Raquel Alves, Takahashi Catarina Satie, Orrico Silvana Regina Perez, Scarel-Caminaga Raquel Mantuaneli
Department of Morphology, School of Dentistry at Araraquara, São Paulo State University (UNESP), 14801-903 Araraquara, SP, Brazil.
Department of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University (UNESP), 14801-903 Araraquara, SP, Brazil.
Mediators Inflamm. 2017;2017:1491405. doi: 10.1155/2017/1491405. Epub 2017 Feb 20.
A high percentage of type 2 diabetes mellitus (T2D) patients are also affected by dyslipidemia and chronic periodontitis (CP), but no studies have determined the gene expression in patients that are simultaneously affected by all three diseases. We investigated the systemic expression of immune-related genes in T2D, dyslipidemia, and CP patients. One hundred and fifty patients were separated into five groups containing 30 individuals each: (G1) poorly controlled T2D with dyslipidemia and CP; (G2) well-controlled T2D with dyslipidemia and CP; (G3) normoglycemic individuals with dyslipidemia and CP; (G4) healthy individuals with CP; (G5) systemic and periodontally healthy individuals. Blood analyses of lipid and glycemic profiles were carried out. The expression of genes, including and was investigated by RT-qPCR. Patients with dyslipidemia demonstrated statistically higher expression of the and genes, while and were lower in comparison with nondyslipidemic patients. Anti-inflammatory genes, such as , positively correlated with parameters of glucose, lipid, and periodontal profiles, while proinflammatory genes, such as , were negatively correlated with these parameters. We conclude that dyslipidemia appears to be the primary disease that is associated with gene expression of immune-related genes, while parameters of T2D and CP were correlated with the expression of these important immune genes.
高比例的2型糖尿病（T2D）患者同时还患有血脂异常和慢性牙周炎（CP），但尚无研究确定同时受这三种疾病影响的患者的基因表达情况。我们调查了T2D、血脂异常和CP患者中免疫相关基因的全身表达情况。150名患者被分为五组，每组30人：（G1）T2D控制不佳合并血脂异常和CP；（G2）T2D控制良好合并血脂异常和CP；（G3）血糖正常合并血脂异常和CP的个体；（G4）患有CP的健康个体；（G5）全身和牙周健康的个体。进行了血脂和血糖谱的血液分析。通过RT-qPCR研究了包括……在内的基因表达。血脂异常患者的……和……基因表达在统计学上显著更高，而与无血脂异常的患者相比，……和……较低。抗炎基因，如……，与血糖、血脂和牙周谱参数呈正相关，而促炎基因，如……，与这些参数呈负相关。我们得出结论，血脂异常似乎是与免疫相关基因表达相关的主要疾病，而T2D和CP的参数与这些重要免疫基因的表达相关。
