Prévost J, Brochu M, Bélanger A, Lambert R
Laboratory of Molecular Endocrinology, Le Centre Hospitalier de l'Université Laval, Québec, Canada.
Gynecol Endocrinol. 1987 Dec;1(4):331-8. doi: 10.3109/09513598709082705.
Concentrations of unconjugated and glucuronated C-21, C-19 and C-18 steroids have been measured in follicular fluid obtained from women hyperstimulated by a combination of clomiphene citrate, pergonal and hCG. Pregnenolone and its glucuronide are found at approximately 5% of the progesterone values (14,395 +/- 2300 ng/ml). Concentrations of dehydroepiandrosterone glucuronide, androst-5-ene-3 beta, 17 beta-diol glucuronide, dihydrotestosterone glucuronide, androstane-3 alpha, 17 beta-diol glucuronide and androstane-3 beta, 17 beta-diol glucuronide are 2-6-fold higher than those of the related unconjugated steroids, while testosterone glucuronide levels are lower than those of testosterone. The concentrations of androsterone glucuronide reach almost 30-fold those of androsterone (2.56 +/- 0.35 ng/ml). By contrast, estradiol and estrone concentrations are 10-20-fold higher than those of conjugated steroids. Measurement of plasma steroid glucuronides indicated that the concentrations in circulation are markedly lower than those detected in follicular fluid. Our data suggest that the ovary in humans may be a site of glucuronidation.
已对接受枸橼酸氯米芬、人绝经期促性腺激素和人绒毛膜促性腺激素联合超刺激的女性卵泡液中未结合及葡萄糖醛酸化的C-21、C-19和C-18甾体浓度进行了测定。孕烯醇酮及其葡萄糖醛酸苷的含量约为孕酮值（14395±2300 ng/ml）的5%。脱氢表雄酮葡萄糖醛酸苷、雄甾-5-烯-3β,17β-二醇葡萄糖醛酸苷、双氢睾酮葡萄糖醛酸苷、雄烷-3α,17β-二醇葡萄糖醛酸苷和雄烷-3β,17β-二醇葡萄糖醛酸苷的浓度比相关未结合甾体的浓度高2至6倍，而睾酮葡萄糖醛酸苷水平低于睾酮。雄酮葡萄糖醛酸苷的浓度几乎达到雄酮浓度（2.56±0.35 ng/ml）的30倍。相比之下，雌二醇和雌酮的浓度比结合甾体的浓度高10至20倍。血浆甾体葡萄糖醛酸苷的测定表明，循环中的浓度明显低于卵泡液中检测到的浓度。我们的数据表明，人类卵巢可能是葡萄糖醛酸化的场所。
