Bélanger A, Couture J, Caron S, Roy R
MRC Group in Molecular Endocrinology, Laval University Medical Center, Ste-Foy, Québec, Canada.
Ann N Y Acad Sci. 1990;595:251-9. doi: 10.1111/j.1749-6632.1990.tb34299.x.
An accurate method is described for analysis of C-21, C-19, and C-18 steroids as well as steroid conjugates, namely, androstane-3 alpha,17 beta-diol glucuronide, androsterone glucuronide, estradiol glucuronide, and estrone glucuronide as well as dehydroepiandrosterone sulfate and androst-5-ene-3 beta,17 beta-diol sulfate. This technique involves an extraction step, aimed at solubilizing the nonconjugated steroids as well as the steroid sulfates and glucuronides, C-18 column chromatography, permitting the separation of nonconjugated steroids and the conjugated group followed by specific hydrolysis of the glucuronide and, finally, solvolysis of the steroid sulfates. Our data indicated that using 1 ml of plasma or 1 g of prostate, good recovery of the three groups of steroids was obtained. Moreover, an accurate determination of steroids could be achieved. The plasma levels of steroids in normal adult women and men found using our technique were within the range of those previously reported by us and other authors.
本文描述了一种用于分析C-21、C-19和C-18类固醇以及类固醇结合物的准确方法，这些结合物包括雄烷-3α,17β-二醇葡萄糖醛酸苷、雄酮葡萄糖醛酸苷、雌二醇葡萄糖醛酸苷、雌酮葡萄糖醛酸苷以及硫酸脱氢表雄酮和雄甾-5-烯-3β,17β-二醇硫酸盐。该技术包括一个提取步骤，旨在溶解未结合的类固醇以及类固醇硫酸盐和葡萄糖醛酸苷，C-18柱色谱法，用于分离未结合的类固醇和结合基团，随后对葡萄糖醛酸苷进行特定水解，最后对类固醇硫酸盐进行溶剂解。我们的数据表明，使用1毫升血浆或1克前列腺组织，可获得三组类固醇的良好回收率。此外，还可实现对类固醇的准确测定。使用我们的技术在正常成年女性和男性中发现的类固醇血浆水平在我们和其他作者先前报道的范围内。
