Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-Cho, Fuchu, Tokyo, 183-8509, Japan.
Vet Res Commun. 2024 Feb;48(1):459-474. doi: 10.1007/s11259-023-10239-2. Epub 2023 Oct 13.
Environmental heat stress has a deleterious impact on farm animal reproductive performance. The purpose of this study was to see how the addition of melatonin affected the efficacy of the superovulation regimen in goats in hot climatic conditions. Sixteen Shiba goats were synchronized and divided into two equal groups (n = 8, each): the melatonin group, which received a single S/C dose of melatonin, and a control group, treated with one ml of corn oil only. Ultrasonographic assessment of ovarian structures (Graafian follicles; GFs and corpus lutea; CLs) morphometry and hemodynamics were performed during the estrous phase of the superovulation (D0) and at day7 after ovulation (D7) of the superovulation regimen. The peripheral reproductive hormones were measured, and microRNAs were characterized. The mean diameter and the total-colored area of GFs during the D0 were significantly (P˂0.05) higher in the melatonin group (5.42 ± 0.11 mm and 1592.20 ± 45.26 pixels, respectively) compared to the control group (4.62 ± 0.12 mm and 1052.55 ± 29.47 pixels, respectively). Concentrations of LH and E increased significantly (P˂0.05) in the melatonin group (1.06 ± 0.06 ng/ml and 46.34 ± 2.77 pg/ml, respectively) compared to the control group (0.75 ± 0.12 ng/ml and 29.33 ± 1.89 pg/ml, respectively). At D7, the melatonin-received goats attained greater values in the mean count (6.75 ± 0.33, P˂0.005), diameters (6.08 ± 0.12 mm, P˂0.01), and total-colored area (17137.30 ± 128.53 pixels, P˂0.01) of detected CLs and progesterone concentrations (4.08 ± 0.24 ng/ml) compared to control goats (4.00 ± 0.28, 4.50 ± 0.19 mm, 11156.87 ± 117.90 pixels, and 2.90 ± 0.18 ng/ml respectively). MiRNA expression analysis was identified during both stages denoting several up and downregulated miRNA candidates among the studied groups. In conclusion, incorporating melatonin enhanced the efficiency of the superovulation regimen in goats under hot climatic conditions.
环境热应激对农场动物的繁殖性能有不良影响。本研究旨在观察添加褪黑素如何影响热环境下山羊超数排卵方案的效果。将 16 只示巴山羊同步并分为两组（n=8，每组）：褪黑素组，单次 S/C 剂量给予褪黑素；对照组，仅给予 1 毫升玉米油。在超数排卵发情期（D0）和超数排卵后第 7 天（D7）进行卵巢结构（格拉夫卵泡；GFs 和黄体；CLs）形态计量学和血液动力学评估。测量外周生殖激素并表征 microRNAs。在 D0 时，褪黑素组的 GF 平均直径和总彩色面积明显（P˂0.05）高于对照组（分别为 5.42±0.11mm 和 1592.20±45.26 像素）。褪黑素组的 LH 和 E 浓度明显（P˂0.05）升高（分别为 1.06±0.06ng/ml 和 46.34±2.77pg/ml），而对照组分别为 0.75±0.12ng/ml 和 29.33±1.89pg/ml。在 D7，接受褪黑素的山羊在检测到的 CLs 的平均计数（6.75±0.33，P˂0.005）、直径（6.08±0.12mm，P˂0.01）和总彩色面积（17137.30±128.53 像素，P˂0.01）以及孕酮浓度（4.08±0.24ng/ml）方面均高于对照组（分别为 4.00±0.28、4.50±0.19mm、11156.87±117.90 像素和 2.90±0.18ng/ml）。在两个阶段的 miRNA 表达分析中都鉴定到了几个上调和下调的 miRNA 候选物。总之，在热环境下，在山羊超数排卵方案中添加褪黑素提高了其效率。
