Institutes of Physical Science and Information Technology, Anhui University, Hefei, Anhui, 230601, P. R. China.
Hefei National Laboratory for Physical Sciences at the Microscale, iChEM (Collaborative Innovation Centre of Chemistry for Energy Materials), CAS Centre for Excellence in Nanoscience, Department of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui, 230026, China.
Sci Rep. 2019 Aug 12;9(1):11640. doi: 10.1038/s41598-019-47660-0.
The fluorescence emission from green fluorescent protein (GFP) is known to be heavily influenced by hydrogen bonding between the core fluorophore and the surrounding side chains or water molecules. Yet how to utilize this feature for modulating the fluorescence of GFP chromophore or GFP-like fluorophore still remains elusive. Here we present theoretical calculations to predict how hydrogen bonding could influence the excited states of the GFP-like fluorophores. These studies provide both a new perspective for understanding the photophysical properties of GFP as well as a solid basis for the rational design of GFP-based fluorophores.
绿色荧光蛋白（GFP）的荧光发射众所周知受到核心荧光团与周围侧链或水分子之间氢键的强烈影响。然而，如何利用这一特性来调节 GFP 发色团或 GFP 类荧光团的荧光仍然难以捉摸。在这里，我们进行了理论计算，以预测氢键如何影响 GFP 类荧光团的激发态。这些研究不仅为理解 GFP 的光物理性质提供了新的视角，也为基于 GFP 的荧光团的合理设计提供了坚实的基础。
