Department of Biotechnology, Chandigarh University, Gharuan, Mohali 140413, Punjab, India; University Centre for Research and Development, Chandigarh University, Gharuan, Mohali 140413, Punjab, India.
Department of Biotechnology, Chandigarh University, Gharuan, Mohali 140413, Punjab, India; University Centre for Research and Development, Chandigarh University, Gharuan, Mohali 140413, Punjab, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Nov 15;321:124752. doi: 10.1016/j.saa.2024.124752. Epub 2024 Jun 27.
Serum creatinine (CR) is regarded as one of the most sought out prognostic biomarkers in medical evaluation of chronic kidney disease (CKD). In light of the diagnostic significance of CR, the utility of a fluorescence biosensor for its detection in human urine specimens has been explored based on Förster resonance energy transfer (FRET) across nitrogen-doped carbon dots (N-CDs) and gold nanoparticles (GNPs). A straightforward microwave-assisted synthesis procedure has been adopted to prepare N-CDs (λ = 400 nm, λ = 540 ± 5 nm) with bright green emissions. On addition of pre-synthesized GNPs, the radiative emanation of the N-CDs is completely suppressed on account of FRET across the N-CDs and the GNPs. About 77 % of their fluorescence intensity is recovered after adding CR to GNPs@N-CDs nanocomposite. The limit of detection for CR sensing is estimated as 0.02 µg•mL. This biosensor is selective enough to recognize CR in the existence of potential interfering substances (e.g., ascorbic acid, glucose, glutathione, urea, and electrolytes). Its practical utility for CR detection has been validated further on the basis of satisfactory correlation with the benchmark Jaffe method, as observed in artificial/human urine specimens. Consequently, this manuscript marks a pioneering report on employing CDs and GNPs-based FRET for identifying CR in urine specimens of CKD patients.
血清肌酐（CR）被认为是慢性肾病（CKD）医学评估中最受关注的预后生物标志物之一。鉴于 CR 的诊断意义，基于氮掺杂碳点（N-CDs）和金纳米粒子（GNPs）之间的Förster 共振能量转移（FRET），已经探索了用于检测人尿液标本中 CR 的荧光生物传感器的实用性。采用简单的微波辅助合成程序制备了具有明亮绿色发射的 N-CDs（λ=400nm，λ=540±5nm）。加入预先合成的 GNPs 后，由于 N-CDs 和 GNPs 之间的 FRET，N-CDs 的辐射发射完全被抑制。在将 CR 添加到 GNPs@N-CDs 纳米复合材料后，其荧光强度恢复了约 77%。CR 传感的检测限估计为 0.02μg•mL。该生物传感器具有足够的选择性，可在存在潜在干扰物质（例如抗坏血酸、葡萄糖、谷胱甘肽、尿素和电解质）的情况下识别 CR。在人工/人尿液标本中与基准 Jaffe 方法观察到的令人满意的相关性进一步验证了其用于 CR 检测的实用性。因此，本文标志着首次使用基于 CDs 和 GNPs 的 FRET 来鉴定 CKD 患者尿液标本中的 CR。
