甲基营养型产甲烷古菌的DNA和RNA稳定同位素探测
DNA and RNA Stable Isotope Probing of Methylotrophic Methanogenic Archaea.
作者信息
Yin Xiuran, Kulkarni Ajinkya C, Friedrich Michael W
机构信息
Microbial Ecophysiology Group, Faculty of Biology/Chemistry, University of Bremen, Bremen, Germany.
MARUM-Center for Marine Environmental Sciences, University of Bremen, Bremen, Germany.
出版信息
Methods Mol Biol. 2019;2046:189-206. doi: 10.1007/978-1-4939-9721-3_15.
Methylotrophic methanogenic archaea are an integral part of the carbon cycle in various anaerobic environments. Different from methylotrophic bacteria, methylotrophic methanogens assimilate both, the methyl compound and dissolved inorganic carbon. Here, we present DNA- and RNA-stable isotope probing (SIP) methods involving an effective labeling strategy using C-labeled dissolved inorganic carbon (DIC) as carbon source along with methanol as dissimilatory substrate.
甲基营养型产甲烷古菌是各种厌氧环境中碳循环不可或缺的一部分。与甲基营养型细菌不同，甲基营养型产甲烷菌既能同化甲基化合物，也能同化溶解的无机碳。在此，我们介绍了DNA和RNA稳定同位素探测（SIP）方法，该方法涉及一种有效的标记策略，即使用13C标记的溶解无机碳（DIC）作为碳源，并以甲醇作为异化底物。
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