a Department of Thoracic Surgery, Huaihe Hospital of Henan University , Kaifeng , Henan , P. R. China.
b College of Nursing and Health, Henan University , Kaifeng , Henan , P. R. China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3410-3416. doi: 10.1080/21691401.2019.1652623.
Non-small-cell lung carcinoma (NSCLC) is the most common type of lung cancer. Circular RNA ZFR (circZFR) is an identified circular RNA (circRNA) that is correlated with cancer progression. However, the role of circZFR in NSCLC remains unknown. In the present study, we aimed to investigate the function of circZFR in NSCLC and the underlying mechanism.
The expression patterns of circZFR were determined using qRT-PCR in NSCLC samples and cell lines. The subcellular distribution of circZFR in NSCLC cells was analyzed by fluorescence hybridization (FISH). Cell proliferation was examined utilizing the CCK-8 assay. Cell migration and invasion were evaluated using the Transwell assay. We used the bioinformatics software TargetScan and miRanda to predict circRNA-miRNA and miRNA-mRNA interactions.
Our results showed that circZFR expressions were significantly upregulated in NSCLC tissues and cell lines. Knockdown of circZFR significantly inhibited the cell proliferation, migration and invasion of NSCLC cells . Furthermore, we demonstrated that circZFR acted as a sponge to absorb microRNA-101-3p (miR-101-3p) and promoted cullin 4B (CUL4B) expression.
Collectively, our results demonstrated that circZFR exhibited a carcinogenic role by sponging miR-101-3p and regulating CUL4B expression in NSCLC. These findings provided evidence for understanding the role of circZFR in NSCLC tumourigenesis.
非小细胞肺癌（NSCLC）是最常见的肺癌类型。环状 RNA ZFR（circZFR）是一种已鉴定的环状 RNA（circRNA），与癌症进展相关。然而，circZFR 在 NSCLC 中的作用尚不清楚。在本研究中，我们旨在研究 circZFR 在 NSCLC 中的功能及其潜在机制。
使用 qRT-PCR 检测 NSCLC 样本和细胞系中 circZFR 的表达模式。使用荧光杂交（FISH）分析 NSCLC 细胞中 circZFR 的亚细胞分布。使用 CCK-8 测定法检测细胞增殖。使用 Transwell 测定法评估细胞迁移和侵袭。我们使用生物信息学软件 TargetScan 和 miRanda 预测 circRNA-miRNA 和 miRNA-mRNA 相互作用。
我们的结果表明，circZFR 在 NSCLC 组织和细胞系中表达明显上调。circZFR 敲低显著抑制 NSCLC 细胞的增殖、迁移和侵袭。此外，我们证明 circZFR 作为 microRNA-101-3p（miR-101-3p）的海绵，促进 cullin 4B（CUL4B）的表达。
总之，我们的研究结果表明，circZFR 通过海绵吸附 miR-101-3p 并调节 NSCLC 中的 CUL4B 表达，发挥致癌作用。这些发现为理解 circZFR 在 NSCLC 肿瘤发生中的作用提供了证据。
