a Department of Thoracic Surgery , First People's Hospital of Yunnan Province , Kunming , China.
b Department of Medical Oncology , First People's Hospital of Yunnan Province , Kunming , China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):1595-1602. doi: 10.1080/21691401.2019.1605371.
Long noncoding RNA, long intergenic non-protein-coding RNA p53-induced transcript (LINC-PINT) was showed to be involved in cancer development. However, the biological effect of LINC-PINT on non-small cell lung cancer (NSCLC) remains unknown. Here, we aimed to investigate the role and underlying mechanism of LINC-PINT in NSCLC. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the level of LINC-PINT in NSCLC tissues and cell lines. Cell counting kit-8 (CCK-8), flow cytometry, migration and transwell invasion assays were used to investigate cell proliferation, cell cycle, cell migration and invasion, respectively. The targets of LINC-PINT were verified by both luciferase reporter assay and RNA immunoprecipitation assay. Tumour xenografts were used to reveal the effect of LINC-PINT on tumourigenesis in vivo. We observed that LINC-PINT expression increased in both NSCLC tissues and cell lines. Function assays exhibited that LINC-PINT reduced NSCLC cell proliferation, cell cycle, cell migration and invasion in vitro. We also indicated that LINC-PINT mediated inhibitory effect on cell proliferation, cell cycle, cell migration and invasion by miR-208a-3p/programmed cell death 4 (PDCD4) in NSCLC cells. These findings indicated that LINC-PINT functions as a tumour-suppressor that exerts important regulatory roles in NSCLC progression by sponging miR-208a-3p/PDCD4.
长链非编码 RNA，长基因间非蛋白编码 RNA p53 诱导转录物（LINC-PINT）被证明参与癌症的发展。然而，LINC-PINT 对非小细胞肺癌（NSCLC）的生物学效应仍不清楚。在这里，我们旨在研究 LINC-PINT 在 NSCLC 中的作用和潜在机制。实时定量聚合酶链反应（qRT-PCR）用于测量 NSCLC 组织和细胞系中 LINC-PINT 的水平。细胞计数试剂盒-8（CCK-8）、流式细胞术、迁移和 Transwell 侵袭实验分别用于研究细胞增殖、细胞周期、细胞迁移和侵袭。通过荧光素酶报告基因检测和 RNA 免疫沉淀实验验证了 LINC-PINT 的靶标。肿瘤异种移植用于揭示 LINC-PINT 在体内对肿瘤发生的影响。我们观察到 LINC-PINT 在 NSCLC 组织和细胞系中的表达均增加。功能分析表明，LINC-PINT 在体外降低了 NSCLC 细胞的增殖、细胞周期、细胞迁移和侵袭。我们还表明，LINC-PINT 通过 miR-208a-3p/程序性细胞死亡 4（PDCD4）在 NSCLC 细胞中对细胞增殖、细胞周期、细胞迁移和侵袭的抑制作用。这些发现表明，LINC-PINT 作为一种肿瘤抑制因子发挥作用，通过海绵 miR-208a-3p/PDCD4 对 NSCLC 进展发挥重要的调节作用。
