McElligott M A, Chaung L Y, Baracos V, Gulve E A
Merck Sharp & Dohme Research Laboratories, Department of Animal Physiology, Rahway, NJ 07065.
Biochem J. 1988 Aug 1;253(3):745-9. doi: 10.1042/bj2530745.
The production of prostaglandins (PG) E2 and F2 alpha and their possible role in regulation of protein turnover in cultured skeletal-muscle cells were examined. Primary chick myoblasts and myotubes, and L8 myotubes, produced PGE2 and PGF2 alpha from endogenous arachidonic acid. PG production by all three cell types was increased manyfold by the addition of exogenous arachidonic acid. Arachidonate-stimulated PG production was inhibited by the addition of indomethacin (0.1 mM). When L8 and chick myotubes were treated with PGE2, PGF2 alpha, arachidonic acid (0.01 mM) or indomethacin (0.1 mM), no significant alterations in rates of protein synthesis or degradation were observed. Rates of protein synthesis and degradation in these cells were responsive to the addition of 10% fetal-bovine serum under identical experimental conditions. Thus, in contrast with incubated adult skeletal muscle, it appears that the production of prostaglandin metabolites from arachidonic acid is unrelated to regulation of protein turnover in cultured muscle cells.
研究了前列腺素（PG）E2和F2α的产生及其在调节培养的骨骼肌细胞蛋白质周转中的可能作用。原代鸡成肌细胞、肌管以及L8肌管可从内源性花生四烯酸产生PGE2和PGF2α。添加外源性花生四烯酸后，这三种细胞类型的PG产生均增加了许多倍。添加吲哚美辛（0.1 mM）可抑制花生四烯酸刺激的PG产生。当用PGE2、PGF2α、花生四烯酸（0.01 mM）或吲哚美辛（0.1 mM）处理L8和鸡肌管时，未观察到蛋白质合成或降解速率有明显变化。在相同实验条件下，这些细胞中的蛋白质合成和降解速率对添加10%胎牛血清有反应。因此，与孵育的成年骨骼肌不同，花生四烯酸产生的前列腺素代谢产物似乎与培养的肌肉细胞中蛋白质周转的调节无关。
