前列腺素对兔子宫颈成纤维细胞胶原酶产生的影响。

Effects of prostaglandins on the production of collagenase by rabbit uterine cervical fibroblasts.

作者信息

Goshowaki H, Ito A, Mori Y

机构信息

Department of Biochemistry, Tokyo College of Pharmacy, Japan.

出版信息

Prostaglandins. 1988 Jul;36(1):107-14. doi: 10.1016/0090-6980(88)90106-2.

DOI:10.1016/0090-6980(88)90106-2

PMID:2845482

Abstract

Effects of prostaglandins on the production of collagenase by rabbit uterine cervical fibroblasts were investigated. Exogenous prostaglandin E2 (PGE2) and PGF2 alpha significantly stimulated the production of collagenase in a dose dependent manner, whereas PGI2 did not. Addition of arachidonic acid in the presence of absence of indomethacin to the cell culture did not show any increase in collagenase production. Recombinant human interleukin-1 (rhIL-1) also promoted the production of cervical collagenase independently of endogenous prostaglandin(s). Furthermore both exogenous PGE2 and PGF2 alpha enhanced the rhIL-1-induced collagenase production whereas PGI2 and/or indomethacin did not. These results suggested that exogenous PGE2 and PGF2 alpha but not endogenous prostaglandin(s) participate in cervical ripening and dilation by enhancing collagenase production by rabbit uterine cervical cells.

摘要

研究了前列腺素对兔子宫颈成纤维细胞胶原酶产生的影响。外源性前列腺素E2（PGE2）和PGF2α以剂量依赖的方式显著刺激胶原酶的产生，而前列环素（PGI2）则无此作用。在细胞培养中添加花生四烯酸，无论有无吲哚美辛，胶原酶的产生均未增加。重组人白细胞介素-1（rhIL-1）也能独立于内源性前列腺素促进宫颈胶原酶的产生。此外，外源性PGE2和PGF2α均增强了rhIL-1诱导的胶原酶产生，而PGI2和/或吲哚美辛则无此作用。这些结果表明，外源性PGE2和PGF2α而非内源性前列腺素通过增强兔子宫颈细胞胶原酶的产生参与宫颈成熟和扩张。