Central Laboratory, The Dongguan Affiliated Hospital of Medical College of Jinan University, The Fifth People's Hospital of Dongguan, Dongguan, China.
Department of Cardiology, The Dongguan Affiliated Hospital of Medical College of Jinan University, The Fifth People's Hospital of Dongguan, Dongguan, China.
J Biomed Mater Res A. 2019 Dec;107(12):2756-2763. doi: 10.1002/jbm.a.36778. Epub 2019 Sep 9.
Poly-l-lactic acid (PLLA) is widely used in clinic, for example, as biodegradable coronary artery stents. However, inflammatory responses in endothelial cells associated with PLLA degradation are relatively undefined. We previously reported inflammation in human aortic endothelial cells (HAEC) in vitro and in vivo. Here, we further assessed inflammatory injury, including cell migration, cell function, and inflammatory cytokines expressed in HAEC treated with PLLA and curcumin by CCK-8, wound healing assay, ELISA, and Western blot. Significant inhibition of cell migration, remarkable dysfunction, and inflammatory responses were found in HAEC treated with PLLA degradation extract, and these effects were alleviated by Cur treatment. These findings indicated that cautious evaluation of biodegradable polymers should be performed, and Cur represents a promising anti-inflammatory agent for alleviating endothelial dysfunction and inflammation caused by PLLA degradation. In addition, Cur should be further studied experimentally in in vivo experiments on animal models as a potential therapeutic to reduce thrombosis of biodegradable polymer stents.
聚左旋乳酸（PLLA）广泛应用于临床，例如作为可生物降解的冠状动脉支架。然而，与 PLLA 降解相关的内皮细胞炎症反应还相对不明确。我们之前曾报道过 PLLA 在体外和体内诱导人主动脉内皮细胞（HAEC）的炎症反应。在这里，我们进一步评估了 HAEC 经 PLLA 和姜黄素处理后的炎症损伤，包括细胞迁移、细胞功能和细胞因子表达，采用 CCK-8 法、划痕愈合实验、ELISA 和 Western blot 检测。发现 PLLA 降解提取物处理的 HAEC 细胞迁移显著抑制，细胞功能显著受损，炎症反应明显，而 Cur 处理可减轻这些影响。这些发现表明，应该谨慎评估可生物降解聚合物，而 Cur 作为一种有前途的抗炎剂，可能减轻由 PLLA 降解引起的内皮功能障碍和炎症。此外，Cur 应该在动物模型的体内实验中进一步研究，作为减少可生物降解聚合物支架血栓形成的潜在治疗方法。
