Plant Protection Department, Faculty of Agriculture, Assiut University, Assiut, Egypt.
Physics Department, Faculty of Science, Assiut University, Assiut, Egypt.
Vector Borne Zoonotic Dis. 2020 Feb;20(2):134-142. doi: 10.1089/vbz.2019.2474. Epub 2019 Aug 13.
The use of conventional pesticides becomes a complicated issue as more species of insect pests become resistant to them. Nanopesticides suit new approaches in pest control. Herein, we tested the toxicological efficacy of imidacloprid compared with three of its nanoformulations (IMD01, IMD02, and IMD03) on second and fourth instar of larvae. Furthermore, we assessed the synergistic actions of piperonyl butoxide (PBO) on imidacloprid and its nanoformulations against second and fourth instar of . The nanoformulation (IMD03) was the most potent insecticide (LC = 14, 6, and 2 ng/mL after 24, 48, and 72 h of exposure, respectively), whereas the lowest toxic nanoformulation was IMD01. However, imidacloprid had the lowest toxicity among the tested compounds (LC = 1015, 705, and 621 ng/mL after 24, 48, and 72 h of exposure, respectively). PBO significantly synergized imidacloprid and its nanoformulations. However, the most synergistic effects were on IMD03 and the lowest was imidacloprid itself. Based on our results, nanopesticides are currently the most promising tool to control mosquitoes. However, further semifield and field studies should be done to illustrate the efficacy of imidacloprid and its nanoformulations on mosquitoes.
随着越来越多的昆虫物种对传统杀虫剂产生抗药性，这些杀虫剂的使用变得更加复杂。纳米农药适合害虫防治的新方法。在此，我们测试了与三种纳米制剂（IMD01、IMD02 和 IMD03）相比，吡虫啉对二龄和四龄幼虫的毒力功效。此外，我们评估了增效醚（PBO）对吡虫啉及其纳米制剂对二龄和四龄幼虫的协同作用。纳米制剂（IMD03）是最有效的杀虫剂（暴露 24、48 和 72 小时后的 LC 分别为 14、6 和 2 ng/mL），而 IMD01 的毒性最低。然而，在测试的化合物中，吡虫啉的毒性最低（暴露 24、48 和 72 小时后的 LC 分别为 1015、705 和 621 ng/mL）。PBO 显著增强了吡虫啉及其纳米制剂的协同作用。然而，协同作用最显著的是 IMD03，而吡虫啉本身的协同作用最低。根据我们的结果，纳米农药是目前控制 蚊子最有前途的工具。然而，应该进行进一步的半田间和田间研究，以说明吡虫啉及其纳米制剂对 蚊子的功效。
