单突触追踪：分步方案
Monosynaptic tracing: a step-by-step protocol.
作者信息
Lavin Thomas K, Jin Lei, Wickersham Ian R
机构信息
McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, United States.
McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, United States.
出版信息
J Chem Neuroanat. 2019 Dec;102:101661. doi: 10.1016/j.jchemneu.2019.101661. Epub 2019 Aug 10.
Abstract
Monosynaptic tracing using deletion-mutant rabies virus allows whole-brain mapping of neurons that are directly presynaptic to a targeted population of neurons. The most common and robust way of implementing it is to use Cre mouse lines in combination with Cre-dependent adeno-associated viral vectors for expression of the required genes in the targeted neurons before subsequent injection of the rabies virus. Here we present a step-by-step protocol for performing such experiments using first-generation (ΔG) rabies viral vectors.
摘要
使用缺失突变型狂犬病病毒进行单突触追踪，能够对直接位于目标神经元群体突触前的神经元进行全脑图谱绘制。实现这一目标最常用且可靠的方法是，在随后注射狂犬病病毒之前，将Cre小鼠品系与依赖Cre的腺相关病毒载体联合使用，以便在目标神经元中表达所需基因。在此，我们提供了一份使用第一代（ΔG）狂犬病病毒载体进行此类实验的详细步骤方案。
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