Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Chemistry and Environmental Engineering, Yancheng Teachers University, Xiwang Road, Yancheng 224007, Jiangsu Province, PR China.
Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Chemistry and Environmental Engineering, Yancheng Teachers University, Xiwang Road, Yancheng 224007, Jiangsu Province, PR China.
J Pharm Biomed Anal. 2019 Nov 30;176:112804. doi: 10.1016/j.jpba.2019.112804. Epub 2019 Aug 5.
In the present study, deep eutectic solvents (DESs) were prepared and firstly applied to extract flavonoids from Safflower using ultrasonic-assisted extraction (UAE), and DES2-UAE was selected as a green and efficient extraction method. The effects of extraction parameters on extraction efficiency (EE) of flavonoids were investigated by single-factor experiments and optimized using a Box-Behnken design (BBD). The optimal results were 56.37 mL/g liquid-solid ratio, 55.85 min extraction time, 41.44 °C extraction temperature and 188.55 W ultrasonic power, respectively. Under optimal extraction conditions, the highest EE of flavonoids (55.41 mg/g) was obtained. The verification experimental results were in good correlation with the predicted results. The adsorption and desorption experiments of flavonoids on five types of macroporous resins (NKA-2, AB-8, HPD-100, D-101 and S-8) were carried out, NKA-2 and S-8 showed higher adsorption/desorption capacities, successively followed by AB-8, D-101 and HPD-100. The adsorption behaviors were better explained by Langmuir isotherm model according to correlation coefficient on the basis of static adsorption test at different temperature. In conclusion, the developed DES2-UAE combined with macroporous resin enrichment can be an alternative method for the green and efficient extraction of bioactive flavonoids from plant materials.
在本研究中，制备了深共晶溶剂（DESs），并首次应用于超声辅助提取（UAE）从红花中提取黄酮类化合物，选择 DES2-UAE 作为一种绿色高效的提取方法。通过单因素实验考察了提取参数对黄酮类化合物提取效率（EE）的影响，并采用 Box-Behnken 设计（BBD）进行优化。最优结果分别为液固比 56.37 mL/g、提取时间 55.85 min、提取温度 41.44°C 和超声功率 188.55 W。在最优提取条件下，黄酮类化合物的最高 EE（55.41 mg/g）。验证实验结果与预测结果吻合良好。对五种大孔树脂（NKA-2、AB-8、HPD-100、D-101 和 S-8）进行了黄酮类化合物的吸附和解吸实验，NKA-2 和 S-8 表现出较高的吸附/解吸能力，其次是 AB-8、D-101 和 HPD-100。根据不同温度下静态吸附试验的相关系数，吸附行为较好地用 Langmuir 等温模型解释。总之，开发的 DES2-UAE 结合大孔树脂富集可以作为从植物材料中绿色高效提取生物活性黄酮类化合物的替代方法。
