Huang Zelin, Zhao Yan, Yang Weixing, Lang Lu, Sheng Jun, Tian Yang, Gao Xiaoyu
Yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming 650201, China.
College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
Food Chem X. 2025 Jan 13;25:102177. doi: 10.1016/j.fochx.2025.102177. eCollection 2025 Jan.
is an important homologous medicinal and food plant, and its fruit is rich in flavonoids. However, few studies have reported the preparation and bioactivity of flavonoids in (ATF). In this study, the optimal conditions for ultrasound-assisted extraction of ATF were identified through response surface optimization. HPD300 was identified as the best resin for the purification of ATF, as it exhibited a Freundlich model-conformative adsorption isotherm. Among the different concentrations of ethanol, 20 % and 30 % resulted in higher flavonoid purity (>90 %) and stronger antioxidant and α-glucosidase inhibition activities. A widely targeted metabolomics assay revealed that the relative abundance of flavonoids in a mixture of 20 % and 30 % ethanol eluates was greater than 73 %, which mainly contained (+)-epicatechin, isoquercitrin, astragalin kaempferol-3-O-rutinoside, and procyanidin B2. These findings provide a theoretical basis for the in-depth development and potential use of ATF in the functional food, cosmetic and pharmaceutical industries.
是一种重要的同源药食两用植物，其果实富含黄酮类化合物。然而，关于[植物名称未给出]（ATF）中黄酮类化合物的制备及生物活性的研究报道较少。在本研究中，通过响应面优化确定了超声辅助提取ATF的最佳条件。HPD300被确定为纯化ATF的最佳树脂，因为它呈现出符合弗罗因德利希模型的吸附等温线。在不同浓度的乙醇中，20%和30%的乙醇洗脱液中黄酮类化合物纯度较高（>90%），且抗氧化和α-葡萄糖苷酶抑制活性较强。广泛靶向代谢组学分析表明，20%和30%乙醇洗脱液混合物中黄酮类化合物的相对丰度大于73%，主要包含（+）-表儿茶素、异槲皮苷、紫云英苷、山柰酚-3-O-芸香糖苷和原花青素B2。这些发现为ATF在功能食品、化妆品和制药行业的深入开发和潜在应用提供了理论依据。
