Department of Civil and Environmental Engineering, Taibah University, Al Medina, Saudi Arabia; London Research and Development Centre, Agriculture and Agri-Food Canada, London, Canada.
Department of Civil Engineering, Lakehead University, Thunder Bay, Canada.
Chemosphere. 2019 Dec;237:124514. doi: 10.1016/j.chemosphere.2019.124514. Epub 2019 Aug 5.
This study investigated the isolation and characterization of three novel bacterial strains; Acinetobacter calcoaceticus, Sphingobacterium multivorum, and Sinorhizobium, isolated form agriculture land. From three hundred strains of bacteria, the three isolates were identified for their superior diesel degradation ability by a series of bench-scale tests. The isolates were further investigated in bench tests for their ability to grow in different diesel fuel concentrations, temperature and pH; degrade diesel fuel in vitro; and for the identification of functional genes. Semi-pilot bioelectrokinetic tests were conducted in three electrokinetic cells. An innovative electrode configuration was adopted to stabilize the soil pH and water content during the test. The genes expressed in the diesel degradation process including Lipases enzymes Lip A, LipB, Alk-b2, rubA, P450, and 1698/2041 were detected in the three isolates. The results showed that the solar panel voltage output is in agreement with the trapezoid model. The temperatures in the cells were found to be 5-7 °C higher than the ambient temperature. The electrode configuration succeeded in stabilizing the soil pH and water content, preventing the development of a pH gradient, important progress for the survival of bacteria. The diesel degradation in the soil after bioelectrokinetic tests were 20-30%, compared to 10-12% in the controls. The study succeeded in developing environmentally friendly technology employing novel bacterial strains to degrade diesel fuel and utilizing solar panels to produce renewable energy for bioelectrokinetics during the winter season.
醋酸钙不动杆菌、多形鞘氨醇单胞菌和中华根瘤菌。从 300 株细菌中，通过一系列台式测试，鉴定出这三种分离物具有卓越的柴油降解能力。在台式测试中，进一步研究了这些分离物在不同柴油燃料浓度、温度和 pH 值下的生长能力、体外降解柴油的能力以及功能基因的鉴定。在三个电动细胞中进行了半中试生物电化学测试。采用了一种创新的电极配置来稳定测试过程中的土壤 pH 值和含水量。在三种分离物中检测到柴油降解过程中表达的基因，包括脂肪酶 Lip A、LipB、Alk-b2、rubA、P450 和 1698/2041。结果表明，太阳能电池板的电压输出与梯形模型一致。发现电池内的温度比环境温度高 5-7°C。电极配置成功地稳定了土壤 pH 值和含水量，防止了 pH 值梯度的发展，这对于细菌的生存是一个重要的进展。与对照相比，生物电化学测试后土壤中的柴油降解率为 20-30%，而对照为 10-12%。本研究成功开发了一种环保技术，利用新型细菌菌株降解柴油，并利用太阳能电池板在冬季为生物电化学过程生产可再生能源。
