Malekshah Rahime Eshaghi, Khaleghian Ali
Department of Chemistry, Semnan University, Semnan, Iran.
Biochemistry Department, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
Drug Res (Stuttg). 2019 Oct;69(11):630-638. doi: 10.1055/a-0975-9124. Epub 2019 Aug 13.
The present study aimed to investigate effects of medicinal plant (Silybum marianum) on the animals with treatment of morphine addiction compared to chemical drug (Naloxane). Also, this study was conducted to evaluate the role of Silybum marianum on factors of serum ALT, AST activities and activity of the antioxidant enzyme:superoxide dismutase (SOD) as well as the extent of lipid peroxidation of Morphine addicted rats.High performance liquid chromatography (HPLC) has been used to measure Morphine in the serum, enzymes functions well as lipid peroxidation of Morphine addicted animals. Results demonstrate treatment with S. Marianum for opium rats at dose 400 mg/kg led to a major reduction in serum morphine compared to Naloxane (400 mg/kg>200 mg/kg>100 mg/kg>Naloxane. The positive effect of dose-dependence on liver enzymes function (ALT and AST) in order of 400 mg/kg>Naloxane>200 mg/kg>100 mg/kg. Furthermore, the findings show that the malondialdehyde levels are increased in opium-treated animals. However, the extracts also demonstrate a significant reduction in the MDA levels compared to the control, 400 mg/kg>200 mg/kg>Naloxane>100 mg/kg. Increase of Silybum marianum extract in rats pretreated significantly elevated the activities of superoxide dismutase (SOD).At last, our data suggest that Silibinin as the main component found in Milk thistle is more selective toward -Opioid Receptor than Morphine and Naloxane as a narcotic receptor antagonist.
本研究旨在调查药用植物（水飞蓟）对吗啡成瘾动物的影响，并与化学药物（纳洛酮）进行比较。此外，本研究还评估了水飞蓟对血清谷丙转氨酶（ALT）、谷草转氨酶（AST）活性以及抗氧化酶超氧化物歧化酶（SOD）活性的影响，以及吗啡成瘾大鼠的脂质过氧化程度。高效液相色谱法（HPLC）已用于测量吗啡成瘾动物血清中的吗啡、酶功能以及脂质过氧化情况。结果表明，与纳洛酮相比，用400mg/kg剂量的水飞蓟治疗鸦片大鼠可导致血清吗啡大幅降低（400mg/kg>200mg/kg>100mg/kg>纳洛酮）。剂量依赖性对肝酶功能（ALT和AST）的积极影响顺序为400mg/kg>纳洛酮>200mg/kg>100mg/kg。此外，研究结果表明，鸦片处理动物的丙二醛水平升高。然而，与对照组相比，提取物也显示丙二醛水平显著降低（400mg/kg>200mg/kg>纳洛酮>100mg/kg）。预先处理大鼠后水飞蓟提取物的增加显著提高了超氧化物歧化酶（SOD）的活性。最后，我们的数据表明，水飞蓟中的主要成分水飞蓟宾对μ-阿片受体的选择性高于吗啡和作为麻醉受体拮抗剂纳洛酮。
