Mengod G, Palacios J M, Probst A, Harris B
Preclinical Research, Sandoz Ltd, Basle, Switzerland.
Brain Res. 1988 Aug;464(1):23-9. doi: 10.1016/0169-328x(88)90014-9.
Using a cDNA probe complementary to an mRNA coding for the alpha-subunit of a human GTP-binding protein that stimulates adenylate cyclase we have studied its regional distribution in human brain by in situ hybridization histochemistry. The specificity of the hybridization signal was examined by using a labelled sense probe and RNase treatment. Gs alpha transcripts presented a widespread but heterogeneous distribution in human brain postmortem tissues. The cell bodies of the granular layer of the cerebellum were the most heavily labelled cells in all the cases examined. High levels of hybridization were also seen in the pyramidal cell layer of the hippocampus and over the cell bodies of the granule cells of the dentate gyrus. Several cortical regions also presented high levels of hybridization. Another area rich in the Gs alpha mRNA was the hypothalamus. The caudate and putamen nuclei presented intermediate levels while the globus pallidus, the thalamus, the midbrain and the brainstem presented only very low levels of hybridization. This distribution differs from the known distribution of adenylate cyclase activity and other GTP-binding proteins, and could indicate that this particular Gs alpha clone codes for a subset of the alpha-subunit of the Gs protein family.
我们使用了一种与编码人类刺激性腺苷酸环化酶的GTP结合蛋白α亚基的mRNA互补的cDNA探针，通过原位杂交组织化学研究了其在人脑内的区域分布。通过使用标记的正义探针和核糖核酸酶处理来检测杂交信号的特异性。Gsα转录本在人脑死后组织中呈现广泛但不均匀的分布。在所有检查的病例中，小脑颗粒层的细胞体是标记最强烈的细胞。在海马锥体细胞层和齿状回颗粒细胞的细胞体上也观察到高水平的杂交。几个皮质区域也呈现高水平的杂交。另一个富含Gsα mRNA的区域是下丘脑。尾状核和壳核呈现中等水平，而苍白球、丘脑、中脑和脑干仅呈现非常低水平的杂交。这种分布与已知的腺苷酸环化酶活性和其他GTP结合蛋白的分布不同，这可能表明这个特定的Gsα克隆编码Gs蛋白家族α亚基的一个子集。
