Largent B L, Jones D T, Reed R R, Pearson R C, Snyder S H
Howard Hughes Medical Institute Laboratory of Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Proc Natl Acad Sci U S A. 1988 Apr;85(8):2864-8. doi: 10.1073/pnas.85.8.2864.
Guanine nucleotide-binding regulatory proteins (G proteins) mediate many receptor-coupled signal transduction events. We have localized in rat brain by in situ hybridization the mRNA for the G protein subunits--G alpha s, G alpha o, and G beta. Oligonucleotide probes were radiolabeled by a technique that resulted in a probe of defined specific activity and uniform length. mRNA species encoding G alpha s and G beta occur in high densities heterogeneously throughout the brain, especially in large neuronal cell bodies--e.g., hippocampal pyramidal cells, granule cells of the dentate gyrus, hypothalamic nuclei, and neurons of brainstem nuclei and the reticular formation. G alpha o mRNA has a more limited distribution and abundance, being detectable in the claustrum, endopiriform nucleus, habenula, hippocampal pyramidal cells, granule cells of the dentate gyrus, and cerebellar Purkinje cells.
鸟嘌呤核苷酸结合调节蛋白（G蛋白）介导许多受体偶联的信号转导事件。我们通过原位杂交在大鼠脑中定位了G蛋白亚基——Gαs、Gαo和Gβ的mRNA。寡核苷酸探针通过一种技术进行放射性标记，该技术产生了具有确定比活性和均匀长度的探针。编码Gαs和Gβ的mRNA种类在整个大脑中高密度地异质分布，特别是在大型神经元细胞体中——例如，海马锥体细胞、齿状回颗粒细胞、下丘脑核以及脑干核和网状结构的神经元。Gαo mRNA的分布和丰度更有限，可在屏状核、内梨状核、缰核、海马锥体细胞、齿状回颗粒细胞和小脑浦肯野细胞中检测到。
