Horne D W, Patterson D
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232.
Clin Chem. 1988 Nov;34(11):2357-9.
Microbiological assay is still widely used for estimating folic acid derivatives in serum and other biological samples. We describe here a modification of this procedure involving use of 96-well microtiter plates. This procedure, used with modern, computer-interfaced microtiter-plate readers and data-reduction software, greatly shortens the time and minimizes reagent costs for this assay. Under the conditions of our assay procedures, all folic acid derivatives tested gave equal growth response for Lactobacillus casei. Results for assays of rat liver extracts showed excellent agreement between the standard bioassay and the 96-well procedure.
微生物测定法仍广泛用于估算血清及其他生物样品中的叶酸衍生物。我们在此描述了该方法的一种改进，涉及使用96孔微量滴定板。该方法与现代的、计算机接口的微量滴定板读数器及数据处理软件一起使用，大大缩短了该测定的时间并将试剂成本降至最低。在我们的测定程序条件下，所有测试的叶酸衍生物对干酪乳杆菌都有相同的生长反应。大鼠肝脏提取物的测定结果表明，标准生物测定法与96孔法之间具有极好的一致性。
