Vasconcelos Maria Elisa Oliveira Costa, Cardoso Andréia Alves, da Silva Josianne Neres, Alexandrino Francisca Jamila Ricarte, Stipp Rafael Nobrega, Nobre-Dos-Santos Marinês, Rodrigues Lidiany Karla Azevedo, Steiner-Oliveira Carolina
Department of Pediatric Dentistry, Piracicaba Dental School, University of Campinas-UNICAMP, Piracicaba, São Paulo, Brazil.
Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas-UNICAMP, Piracicaba, São Paulo, Brazil.
Photobiomodul Photomed Laser Surg. 2019 Sep;37(9):567-573. doi: 10.1089/photob.2019.4669. Epub 2019 Aug 14.
This study aimed to investigate if β-cyclodextrin nanoparticles potentiate the photodynamic antimicrobial chemotherapy (PACT) effects in single and microcosm oral biofilms using methylene blue (MB) and a red laser. Studies of PACT have demonstrated promising effects; however, the association of nanoparticles with photosensitizers could enhance the antimicrobial result. Biofilms were grown on enamel blocks either with or in a microcosm model (salivary microorganisms) supplemented with sucrose. PACT using 50 μM MB associated or not with 32 μM encapsulated β-cyclodextrin with MB for 5 min, followed by irradiation with red laser ( = 660 nm, 320 J/cm), was conducted and the counts of viable microorganisms in proper selective media were determined. Data were analyzed by one-factor ANOVA, followed by Tukey's test, or Kruskal-Wallis test, followed by Dunn's post hoc test, all with a significance level of 5%. In the single-species biofilm model, a significant reduction in counts was found for all groups when light was present. In the microcosm biofilm model, no significant difference was found among the groups for total streptococci, but a significant reduction of was observed for the PACT group of encapsulated β-cyclodextrin+MB. However, no statistically significant difference was observed among the PACT groups. PACT with β-cyclodextrin mediated with MB associated with a red laser reduced in microcosm biofilms. However, the presence of β-cyclodextrin nanoparticles did not potentiate the PACT effects in single or microcosm oral biofilms.
本研究旨在调查β-环糊精纳米颗粒是否能增强使用亚甲蓝（MB）和红色激光的光动力抗菌化疗（PACT）对单一和微观口腔生物膜的作用。PACT的研究已证明有显著效果；然而，纳米颗粒与光敏剂的结合可能会增强抗菌效果。生物膜在添加或不添加蔗糖的釉质块上生长，或在微观模型（唾液微生物）中生长。使用50μM MB联合或不联合32μM包封有MB的β-环糊精处理5分钟，然后用红色激光（λ = 660nm，320J/cm²）照射，进行PACT，并在适当的选择性培养基中测定活菌数。数据通过单因素方差分析，随后进行Tukey检验，或Kruskal-Wallis检验，随后进行Dunn事后检验，所有检验的显著性水平均为5%。在单物种生物膜模型中，光照存在时所有组的菌数均显著减少。在微观生物膜模型中，各组总链球菌之间未发现显著差异，但包封有β-环糊精 + MB的PACT组观察到显著减少。然而，PACT组之间未观察到统计学上的显著差异。MB介导的β-环糊精与红色激光联合的PACT可减少微观生物膜中的菌数。然而，β-环糊精纳米颗粒的存在并未增强PACT对单一或微观口腔生物膜的作用。
