Discipline of Pediatric Dentistry, Faculty of Dentistry, Federal University of Uberlândia, Avenida Pará, 1720, Bloco 2G, Sala 02, Campus Umuarama, 38405-320, Uberlândia, Minas Gerais, Brazil.
Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Avenida Dr. Octávio Pinheiro Brisolla, 9-75 - Vila Universitária, 17012-901, Bauru, São Paulo, Brazil.
Photodiagnosis Photodyn Ther. 2018 Mar;21:163-167. doi: 10.1016/j.pdpdt.2017.12.005. Epub 2017 Dec 9.
Antimicrobial Photodynamic Therapy (aPDT) could enhance the prevention of dental caries lesions in pits and fissures of partially erupted molars, by killing microorganisms from complex dental biofilms. This pilot study aimed to evaluate the effect of Antimicrobial Photodynamic Therapy (aPDT) on the viability of specific microorganism groups of dental microcosm biofilms from occlusal surfaces of first permanent molars in eruption.
Dental microcosm biofilms grown on bovine enamel blocks, from dental plaque collected on occlusal surfaces of a partially erupted lower right first permanent molar, with McBain medium plus 1% sucrose in anaerobic condition at 37 °C for 72 h. The experiments were performed in eight groups: L-P- = no treatment (control), L18.75P- = 18.75 J/cm LED, L37.5P- = 37.5 J/cm LED, L75P- = 75 J/cm LED, L-P+ = 200 mM TBO, L18.75P+ = 200 mM TBO + 18.75 J/cm LED, L37.5P+ = 200 mM TBO + 37.5 J/cm LED, and L75P+ = 200 mM TBO + 75 J/cm LED. The counts of total microorganisms, total streptococci and mutans streptococci were determined on selective media agar plates by colony-forming units per mL. The log-transformed counts were analyzed by Kruskal-Wallis and post-hoc Dunn's test (P < 0.05).
The counts of all microorganisms treated in the group L75P+ were statistically lower than those treated in L-P-. The aPDT promoted a significant reduction of microorganisms, with a trend of dose-dependent effect.
TBO-mediated aPDT was effective in reducing the viability of specific microbial groups in dental microcosm biofilms originated from occlusal of permanent molars in eruption.
抗菌光动力疗法（aPDT）可以通过杀死复杂牙菌斑中的微生物来增强对部分萌出磨牙窝沟龋损的预防作用。本初步研究旨在评估抗菌光动力疗法（aPDT）对萌出下颌第一恒磨牙咬合面牙菌斑来源的牙菌斑微生物群的特定微生物群的活力的影响。
在厌氧条件下，将从部分萌出的下颌右第一恒磨牙咬合面收集的牙菌斑接种在牛牙釉质块上，在 37°C 下使用 McBain 培养基加 1%蔗糖培养 72 小时，形成牙菌斑微宇宙生物膜。实验分为 8 组：L-P-（无处理对照）、L18.75P-（18.75 J/cm LED）、L37.5P-（37.5 J/cm LED）、L75P-（75 J/cm LED）、L-P+（200 mM TBO）、L18.75P+（200 mM TBO+18.75 J/cm LED）、L37.5P+（200 mM TBO+37.5 J/cm LED）和 L75P+（200 mM TBO+75 J/cm LED）。通过菌落形成单位每毫升在选择性琼脂平板上确定总微生物、总链球菌和变异链球菌的数量。对数转换后的计数采用 Kruskal-Wallis 和事后 Dunn 检验（P<0.05）进行分析。
与 L-P-组相比，L75P+组处理的所有微生物的数量均具有统计学意义上的降低。aPDT 显著降低了微生物的数量，且呈剂量依赖性效应。
TBO 介导的 aPDT 可有效降低萌出磨牙咬合面牙菌斑微生物群的特定微生物群的活力。
