Department of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, United States.
Image and Data Analysis Core, Harvard Medical School, Boston, United States.
Elife. 2019 Aug 14;8:e46464. doi: 10.7554/eLife.46464.
The dorsal raphe nucleus (DRN) is an important source of neuromodulators and has been implicated in a wide variety of behavioral and neurological disorders. The DRN is subdivided into distinct anatomical subregions comprised of multiple cell types, and its complex cellular organization has impeded efforts to investigate the distinct circuit and behavioral functions of its subdomains. Here we used single-cell RNA sequencing, in situ hybridization, anatomical tracing, and spatial correlation analysis to map the transcriptional and spatial profiles of cells from the mouse DRN. Our analysis of 39,411 single-cell transcriptomes revealed at least 18 distinct neuron subtypes and 5 serotonergic neuron subtypes with distinct molecular and anatomical properties, including a serotonergic neuron subtype that preferentially innervates the basal ganglia. Our study lays out the molecular organization of distinct serotonergic and non-serotonergic subsystems, and will facilitate the design of strategies for further dissection of the DRN and its diverse functions.
中缝背核（DRN）是神经调质的重要来源，与多种行为和神经紊乱相关。DRN 可细分为不同的解剖亚区，包含多种细胞类型，其复杂的细胞组织阻碍了对其亚区的不同回路和行为功能的研究。在这里，我们使用单细胞 RNA 测序、原位杂交、解剖追踪和空间相关分析来绘制来自小鼠 DRN 的细胞的转录组和空间图谱。我们对 39411 个单细胞转录组的分析揭示了至少 18 种不同的神经元亚型和 5 种 5-羟色胺能神经元亚型，具有不同的分子和解剖特性，包括一种优先支配基底神经节的 5-羟色胺能神经元亚型。我们的研究阐述了不同的 5-羟色胺能和非 5-羟色胺能亚系统的分子组织，并将有助于设计进一步剖析 DRN 及其多种功能的策略。
