乙酰胆碱刺激下单个胰腺腺泡细胞胞质钙的振荡。

Oscillations of cytosolic calcium in single pancreatic acinar cells stimulated by acetylcholine.

作者信息

Yule D I, Gallacher D V

机构信息

Physiological Laboratory, University of Liverpool, England.

出版信息

FEBS Lett. 1988 Nov 7;239(2):358-62. doi: 10.1016/0014-5793(88)80951-7.

DOI:10.1016/0014-5793(88)80951-7

PMID:3141216

Abstract

The changes in cytosolic free calcium concentration [( Ca2+]i) were monitored (fura-2) in single, isolated, mouse pancreatic acinar cells stimulated by acetylcholine (ACh). Responses to ACh at concentrations between 10(-7) and 5 x 10(-7) M are marked by the appearance of regular, sinusoidal, oscillations in [Ca2+]i. At 37 degrees C the oscillations are transient, being seen only in the initial rising phase of the calcium signal. At 30 degrees C regular oscillations can be maintained throughout the period of ACh application. This study reports that release of intracellular calcium and influx of extracellular calcium are both involved in the generation of these oscillatory calcium signals.

摘要

在分离的单个小鼠胰腺腺泡细胞中，利用fura - 2监测由乙酰胆碱（ACh）刺激引起的胞质游离钙浓度[（Ca2 +）i]的变化。在浓度为10（-7）至5×10（-7）M之间的ACh刺激下，[Ca2 +]i会出现规则的正弦振荡。在37℃时，振荡是短暂的，仅出现在钙信号的初始上升阶段。在30℃时，在ACh作用的整个期间都能维持规则振荡。本研究报告称，细胞内钙的释放和细胞外钙的内流都参与了这些振荡性钙信号的产生。