Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
State Key Laboratory of Dao-di Herbs Breeding Base, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
Life Sci. 2019 Sep 15;233:116749. doi: 10.1016/j.lfs.2019.116749. Epub 2019 Aug 11.
Functional dyspepsia (FD) is very common worldwide with a high prevalence of 10%-30%, and it becomes a heavy burden to patients because of its hard to be cured. In our previous study, phenylethanoid glycosides were found to exist in Houpo, a traditional Chinese medicine commonly used for the treatment of abdominal distention, pain and dyspepsia. In the present study, the effect of magnoloside A (MA), a main phenylethanoid glycoside in Houpo, on FD was firstly evaluated and its potential mechanism was concluded.
MA was orally administered consequently for 3 weeks, and its effect on a FD rat model established through transient neonatal gastric irritation and mature alternate-day fasting was tested. Levels of brain-gut peptides and inflammatory factors in blood or tissues were determined by ELISA methods. Meanwhile, the gut microbiota was analyzed by 16S rRNA gene sequencing and short chain fat acids were determined by GC/MS.
MA exhibited anti-FD activities by fastening the delayed gut emptying rate of FD rat and increasing the levels of gastrin, motilin, and calcitonin gene related protein; and decreasing the levels of 5-hydroxytryptamine, nitric oxide synthase, and vasoactive intestinal peptide. On the other hand, MA can modulate the composition of gut microbiota, resulting in the variation of the short chain fat acids.
MA ameliorated FD rats by modulating of the secretion of related brain-gut peptides and altering the composition of intestinal microbiota.
功能性消化不良（FD）在全球范围内非常普遍，患病率高达 10%-30%，由于难以治愈，给患者带来了沉重的负担。在我们之前的研究中，发现厚朴中存在苯乙醇苷，厚朴是一种常用于治疗腹胀、疼痛和消化不良的中药。在本研究中，首次评估了厚朴中的主要苯乙醇苷——木兰脂素 A（MA）对 FD 的作用，并总结了其潜在机制。
MA 连续口服 3 周，检测其对通过短暂新生期胃刺激和成熟隔日禁食建立的 FD 大鼠模型的影响。通过 ELISA 方法测定血液或组织中脑肠肽和炎症因子的水平。同时，通过 16S rRNA 基因测序分析肠道微生物群，通过 GC/MS 测定短链脂肪酸。
MA 通过加速 FD 大鼠的胃排空延迟率，增加胃泌素、胃动素和降钙素基因相关肽的水平，降低 5-羟色胺、一氧化氮合酶和血管活性肠肽的水平，表现出抗 FD 活性。另一方面，MA 可以调节肠道微生物群的组成，导致短链脂肪酸的变化。
MA 通过调节相关脑肠肽的分泌和改变肠道微生物群的组成来改善 FD 大鼠。
