Department of Environmental Science and Engineering, Beijing University of Chemical Technology, No. 15 Beisanhuan East Road, Chaoyang District, Beijing 100029, PR China; Faculty of Water Supply and Environmental Engineering, Arba Minch University, P.O. Box 21, Arba Minch, Ethiopia.
Department of Environmental Science and Engineering, Beijing University of Chemical Technology, No. 15 Beisanhuan East Road, Chaoyang District, Beijing 100029, PR China.
Sci Total Environ. 2019 Oct 15;687:1274-1284. doi: 10.1016/j.scitotenv.2019.05.411. Epub 2019 May 30.
Anaerobic digestion (AD) technology is one of the best eco-friendly waste-to-energy processes. Hence the trend of biogas production shows considerable ranges of fluctuations in entire digestion period. This study analyzes the daily substance conversion dynamics, biomethane production and changes in microbial community structure. The results show that in the first peak of biogas production during 1st up to 4th days of the digestion period, CO was the dominant component. However, the daily methane production (DMP) reveal accelerated increment starting from day 8 up to the peak point on day 13 (462.11 mL/d). The concentration of acetic acid covered 21.80% up to 62.00% of the total VFAs in the first 10 days of digestion period. On the other hand, the accumulation of propionic acid is in the range of 1735.70 mg/L- 2893.12 mg/L in between day 5-15, which is beyond the inhibition level (1000 mg/L) but the system didn't stop biogas production. The distribution of bacterial family such as Clostridiaceae_1, Prevotellaceae, Enterobacteriaceae and Peptostreptococcaceae are the dominant group at early stage as compared to composition in remaining stages except Enterobacteriaceae which have marginally high abundance in lowest biogas production point. The archaeal genus Methanosaeta is dominant among the samples collected at early stages (65.66%-77.22%). However, the Methanobacterium is predominant (34.88%-59.40%) in samples obtained at late stages of AD period. On the other hand, the distribution of Methanosarcina is comparable in the first three samples (S1(16.60%), S2(22.21%) and S3(20.38%)) than the stable stage (S4 (6.7%)). This study demonstrates the detailed conditions at fluctuating and constant biogas production periods, which would benefit future researchers working in similar area.
厌氧消化（AD）技术是一种环保的废物能源化工艺。因此，沼气生产的趋势在整个消化周期内表现出相当大的波动范围。本研究分析了每日物质转化动态、生物甲烷产量和微生物群落结构变化。结果表明，在消化期第 1 至 4 天的第 1 个沼气生产高峰期间，CO 是主要成分。然而，从第 8 天开始，每日甲烷产量（DMP）开始加速增长，直到第 13 天（462.11 毫升/天）达到峰值。在消化期的前 10 天，乙酸浓度占总 VFA 的 21.80%至 62.00%。另一方面，丙酸的积累在第 5 天至第 15 天之间的范围为 1735.70 毫克/升至 2893.12 毫克/升，超过了抑制水平（1000 毫克/升），但系统并未停止沼气生产。与其余阶段相比，细菌家族如 Clostridiaceae_1、Prevotellaceae、Enterobacteriaceae 和 Peptostreptococcaceae 的分布在早期是主要群体，除了 Enterobacteriaceae 在沼气产量最低的点具有较高的丰度外。在早期（65.66%-77.22%）采集的样本中，甲烷八叠球菌属是主要的古菌属。然而，在 AD 期的后期阶段（34.88%-59.40%），甲烷杆菌属是主要的（34.88%-59.40%）。另一方面，在第 1 至 3 个样本（S1(16.60%)、S2(22.21%)和 S3(20.38%))中，甲烷八叠球菌属的分布与稳定阶段（S4(6.7%））相当。本研究展示了波动和稳定沼气生产期的详细条件，这将有利于未来在类似领域工作的研究人员。
