Teixeira Hax Lucas, Rincón Joao Alveiro Alvarado, Schneider Augusto, Pegoraro Lígia Margareth Cantarelli, Franco Collares Letícia, Alves Pereira Rubens, Pradieé Jorgea, Del Pino Francisco Augusto Burket, Nunes Corrêa Marcio
Universidade Federal de Pelotas, Pelotas, RS, 96010-000, Brazil.
Empresa Brasileira de Pesquisa Agropecuária, Pelotas, RS, 96010-971, Brazil.
Zygote. 2019 Oct;27(5):321-328. doi: 10.1017/S0967199419000327. Epub 2019 Aug 15.
Around 60-80% of oocytes maturated in vivo reached competence, while the proportion of maturation in vitro is rarely higher than 40%. In this sense, butafosfan has been used in vivo to improve metabolic condition of postpartum cows, and can represent an alternative to increase reproductive efficiency in cows. The aim of this study was to evaluate the addition of increasing doses of butafosfan during oocyte maturation in vitro on the initial embryo development in cattle. In total, 1400 cumulus-oocyte complexes (COCs) were distributed in four groups and maturated according to supplementation with increasing concentrations of butafosfan (0 mg/ml, 0.05 mg/ml, 0.1 mg/ml and 0.2 mg/ml). Then, 20 oocytes per group were collected to evaluate nuclear maturation and gene expression on cumulus cells and oocytes and the remaining oocytes were inseminated and cultured until day 7, when blastocysts were collected for gene expression analysis. A dose-dependent effect of butafosfan was observed, with decrease of cleavage rate and embryo development with higher doses. No difference between groups was observed in maturation rate and expression of genes related to oocyte quality. Our results suggest that butafosfan is prejudicial for oocytes, compromising cleavage and embryo development.
在体内成熟的卵母细胞中，约60 - 80%达到了成熟能力，而体外成熟的比例很少高于40%。从这个意义上说，布他磷已被用于产后奶牛的体内，以改善其代谢状况，并可作为提高奶牛繁殖效率的一种替代方法。本研究的目的是评估在牛卵母细胞体外成熟过程中添加不同剂量布他磷对早期胚胎发育的影响。总共1400个卵丘-卵母细胞复合体（COCs）被分为四组，并根据布他磷浓度的增加（0毫克/毫升、0.05毫克/毫升、0.1毫克/毫升和0.2毫克/毫升）进行补充培养使其成熟。然后，每组收集20个卵母细胞来评估核成熟情况以及卵丘细胞和卵母细胞中的基因表达，其余的卵母细胞进行授精并培养至第7天，此时收集囊胚进行基因表达分析。观察到布他磷有剂量依赖性效应，随着剂量增加，卵裂率和胚胎发育率降低。在成熟率和与卵母细胞质量相关的基因表达方面，各组之间未观察到差异。我们的结果表明，布他磷对卵母细胞有损害作用，会影响卵裂和胚胎发育。
