Animal Biotechnology Laboratory, Facultad de Agronomía, INPA-CONICET, Buenos Aires University, Buenos Aires, Argentina; Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria Mercedes, Corrientes, Argentina.
Animal Biotechnology Laboratory, Facultad de Agronomía, INPA-CONICET, Buenos Aires University, Buenos Aires, Argentina.
Theriogenology. 2020 May;148:140-148. doi: 10.1016/j.theriogenology.2020.02.045. Epub 2020 Mar 3.
The molecule Dimethyl sulfoxide is widely used as drug solvent. However, its antioxidant property was poorly explored. In this study, we evaluated the effect of DMSO supplementation during oocyte in vitro maturation (IVM) on embryo development and quality. Bovine oocytes were matured with different DMSO concentrations (0, 0.1, 0.25, 0.5, 0.75, 1 and 10% v:v) followed by in vitro fertilization. Subsequently, quality indicators such as gene expression of SOX2, OCT4, CDX2, SOD1, oocyte and embryo redox status and DNA damage were evaluated. Polar body extrusion and blastocyst rates increased with 0.5% v:v DMSO. Moreover, first polar body extrusion and blastocyst rates did not increase with 1%, and 10% of DMSO reduced polar body extrusion and did not produce blastocyst. Optimal concentration of DMSO for the use on the maturation was estimated at around 0.45% v:v. Supplementation with 0.5% v:v DMSO has not affected mRNA abundance of genes key in blastocyst, however 0.75% increased gene expression of OCT4 and SOX2. Oocytes matured with 0.5% v:v DMSO and blastocyst from DMSO group showed reduced lipid peroxidation respect control. Total Glutathione concentrations increased in blastocyst stage in DMSO group. DMSO increased the total cell number of blastocysts but not TUNEL positive cells. In conclusion, our results suggest that low DMSO concentrations used during bovine oocytes in vitro maturation increases the maturation, as well as the blastocyst rate and its quality, without demonstrating deleterious effect on embryo cells.
二甲基亚砜（DMSO）广泛用作药物溶剂。然而，其抗氧化特性尚未得到充分探索。在这项研究中，我们评估了在卵母细胞体外成熟（IVM）期间补充 DMSO 对胚胎发育和质量的影响。用不同浓度的 DMSO（0、0.1、0.25、0.5、0.75、1 和 10%v:v）成熟牛卵母细胞，然后进行体外受精。随后，评估了基因表达（SOX2、OCT4、CDX2、SOD1）、卵母细胞和胚胎氧化还原状态以及 DNA 损伤等质量指标。随着 0.5%v:v DMSO 的加入，极体排出率和囊胚率增加。此外，1%和 10%的 DMSO 并未增加第一极体排出率和囊胚率，反而降低了极体排出率且没有产生囊胚。DMSO 用于成熟的最佳浓度估计在 0.45%v:v 左右。添加 0.5%v:v DMSO 并未影响囊胚关键基因的 mRNA 丰度，但 0.75%的 DMSO 增加了 OCT4 和 SOX2 的基因表达。用 0.5%v:v DMSO 成熟的卵母细胞和来自 DMSO 组的囊胚显示出脂质过氧化反应减少。DMSO 组囊胚阶段的总谷胱甘肽浓度增加。DMSO 增加了囊胚的总细胞数，但没有增加 TUNEL 阳性细胞数。总之，我们的研究结果表明，在牛卵母细胞体外成熟过程中使用低浓度的 DMSO 可提高成熟率和囊胚率及其质量，而对胚胎细胞没有显示出有害影响。
