Suárez-Vega Dubraska V, Velazco de Maldonado Gladys J, Ortíz Reynaldo L, García-Guevara Víctor J, Miller-Kobisher Blanca
Department of Investigation, Faculty of Dentistry, University of Los Andes (ULA), Mérida, Venezuela.
Department of Restorative Dentistry, Faculty of Dentistry, Dental Research Center, University of Los Andes (ULA), Mérida, Venezuela.
J Cutan Aesthet Surg. 2019 Apr-Jun;12(2):145-148. doi: 10.4103/JCAS.JCAS_150_18.
Recently, some clinicians have proposed implanting polydioxanone (PDO) threads imbibed in hyaluronic acid (HA), arguing that this may reinforce the lifting effects. However, this is controversial because PDO sutures are hydrophilic and the presence of HA could increase the rate of hydrolysis. The aim of this study was to demonstrate the degradation of PDO lifting threads in HA through ultramicroscopy. It was a qualitative research and preclinical trial. Three, 1-cm-long, segments of 23-G PDO threads were immersed in 1.5-mL non-crosslinked HA in previously labeled, sterile microcentrifuge tubes. These were observed by ultramicroscopy at 4× and 10× after 24, 48, and 72 h. Microphotographs taken after 24 h show structural changes in the fibers, presenting an increase in interlaminar spaces and dilution of violet pigmentation. At 48 h, degradation continues. PDO hygroscopy is observed as aqueous content between the peripheral layers and the central core of the thread. At 72 h, as the pigment is released, larger empty spaces are observed in the central column of the thread, and there is disorganization of the peripheral fibrils with fraying all along the fiber. HA induces rapid biodegradation of the PDO thread by hydrolysis beginning 24 h after contact of the thread with the biomaterial. The non-crosslinked HA is a powerful catalyzing agent for hydrolytic degradation of the PDO thread, because this thread is highly hydrophilic. Clinically, embedding PDO threads in HA accelerates biodegradation of the suture.
最近，一些临床医生提议植入浸有透明质酸（HA）的聚二氧六环酮（PDO）线，认为这可能会增强提拉效果。然而，这存在争议，因为PDO缝线具有亲水性，而HA的存在可能会加快水解速率。本研究的目的是通过超微显微镜观察来证明HA中PDO提拉线的降解情况。这是一项定性研究和临床前试验。将三段1厘米长的23G PDO线浸入预先标记好的无菌微量离心管中的1.5毫升非交联HA中。在24、48和72小时后，通过4倍和10倍超微显微镜对其进行观察。24小时后拍摄的显微照片显示纤维结构发生变化，层间间隙增大，紫色色素变淡。48小时时，降解仍在继续。观察到PDO吸湿现象，表现为线的外周层和中心芯之间存在含水量。72小时时，随着色素释放，在线的中心柱中观察到更大的空隙，外周原纤维出现紊乱，纤维全长出现磨损。HA在与生物材料接触24小时后通过水解作用诱导PDO线快速生物降解。非交联HA是PDO线水解降解的强力催化剂，因为这种线具有高度亲水性。临床上，将PDO线埋入HA中会加速缝线的生物降解。
