Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine, Seoul National University Bundang Hospital, 82 Gumi-ro 173beon-gil, Bundang-gu, Seongnam, 463-707, Korea.
Jinwoo Bio Co. Ltd., Yongin si, Korea.
Aesthetic Plast Surg. 2024 Jan;48(2):221-227. doi: 10.1007/s00266-023-03614-6. Epub 2023 Aug 29.
Although various products are commonly used for skin rejuvenation, solid-type hyaluronic acid (HA) as an injectable form has not been researched or utilized. This study aimed to demonstrate the safety and efficacy of solid-type HA in thread form, which differs from the conventional gel-type HA commonly used.
Solid-type HA threads, conventional HA fillers, and polydioxanone (PDO) threads were inserted into the dorsal subcutaneous layer of mice. Photographs were taken on days 0, 1, 3, and 7, and on day 7, the samples were harvested for histological analysis. Inflammatory reactions and detection of collagen were confirmed through tissue staining, and real-time PCR was conducted to quantify collagen synthesis.
In the histological analysis, the PDO threads exhibited a greater inflammatory response compared to the HA threads. Masson's trichrome staining revealed a higher degree of collagen synthesis in the HA thread group compared to the HA filler group. While collagen type 1 expression was significantly higher in the PDO thread group than in the HA thread group, the HA thread group showed higher expression levels of collagen type 3. Furthermore, the PDO thread group demonstrated a statistically significant increase in TGF-β1 compared to the HA group.
This in vivo study demonstrated the stable application of solid-type pure HA threads and their potential for inducing collagen production, while also yielding a low inflammatory response. The findings highlight the promising applications of solid-type HA in the field of cosmetic dermatology.
This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
尽管有各种产品常用于皮肤年轻化，但作为注射形式的固态透明质酸（HA）尚未被研究或应用。本研究旨在展示固态 HA 线体形式的安全性和有效性，它与常用的传统凝胶型 HA 不同。
将固态 HA 线体、传统 HA 填充物和聚二氧杂环己酮（PDO）线体插入小鼠背部皮下层。在第 0、1、3 和 7 天拍摄照片，并在第 7 天采集样本进行组织学分析。通过组织染色确认炎症反应和胶原检测，并通过实时 PCR 定量胶原蛋白合成。
在组织学分析中，PDO 线体比 HA 线体表现出更强的炎症反应。Masson 三色染色显示，HA 线体组的胶原合成程度高于 HA 填充物组。虽然 PDO 线体组的 I 型胶原表达明显高于 HA 线体组，但 HA 线体组的 III 型胶原表达水平更高。此外，PDO 线体组 TGF-β1 的表达水平明显高于 HA 组。
这项体内研究表明，固态纯 HA 线体的稳定应用及其诱导胶原生成的潜力，同时产生低炎症反应。这些发现突显了固态 HA 在美容皮肤科领域的应用前景。
