卤虫与大鼠肝脏40S核糖体蛋白的比较研究：用高效液相色谱法对氨基酸组成进行微观分析。
A comparative study on 40S ribosomal proteins of Artemia salina and rat liver: micro analysis of amino acid composition by high-performance liquid chromatography.
作者信息
Odani S, Kenmochi N, Ogata K
机构信息
Department of Biochemistry, Niigata University School of Medicine.
出版信息
J Biochem. 1988 May;103(5):872-7. doi: 10.1093/oxfordjournals.jbchem.a122364.
The amino acid compositions of 24 proteins of 40S ribosomal subunits of Artemia salina cysts were determined and compared with those of rat liver. The basic proteins of A. salina 40S ribosomes were separated by two-dimensional polyacrylamide gel electrophoresis and extracted with 70% formic acid. Samples were freed from contaminants by gel-filtration through a high-performance liquid chromatography column. Amino acid compositions were determined for individual proteins by pre-column derivatization with N,N-dimethylaminoazobenzenesulfonyl chloride followed by reverse phase high-performance liquid chromatography. The similarity of amino acid compositions between A. salina and rat liver 40S ribosomal proteins was evaluated by the method of Cornish-Bowden (Cornish-Bowden, A. (1980) Anal. Biochem. 105, 233-238), and possible relationships between A. salina and rat were detected for 16 protein species (S2, S3, S4, S6, S7, S8, S15a, S16, S17, and S18, strongly related and S14, S15, S20, S23, S24, and S26, weakly related), indicating a conservative nature of eukaryotic ribosomal proteins.
测定了卤虫囊肿40S核糖体亚基的24种蛋白质的氨基酸组成，并与大鼠肝脏的进行了比较。通过二维聚丙烯酰胺凝胶电泳分离卤虫40S核糖体的碱性蛋白质，并用70%甲酸提取。通过高效液相色谱柱进行凝胶过滤，使样品去除污染物。通过用N，N-二甲基氨基偶氮苯磺酰氯进行柱前衍生化，然后进行反相高效液相色谱，测定单个蛋白质的氨基酸组成。采用Cornish-Bowden方法（Cornish-Bowden，A.（1980）Anal.Biochem.105，233-238）评估了卤虫和大鼠肝脏40S核糖体蛋白质之间氨基酸组成的相似性，检测到卤虫和大鼠之间16种蛋白质（S2、S3、S4、S6、S7、S8、S15a、S16、S17和S18，强相关；S14、S15、S20、S23、S24和S26，弱相关）之间可能存在的关系，表明真核核糖体蛋白质具有保守性。
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