真核生物核糖体蛋白的分离。40S核糖体亚基蛋白S2、S3、S4、S5、S6、S7、S8、S9、S13、S23/S24、S27和S28的纯化与特性分析。
The isolation of eukaryotic ribosomal proteins. The purification and characterization of the 40 S ribosomal subunit proteins S2, S3, S4, S5, S6, S7, S8, S9, S13, S23/S24, S27, and S28.
作者信息
Collatz E, Wool I G, Lin A, Stöffler G
出版信息
J Biol Chem. 1976 Aug 10;251(15):4666-72.
The proteins of the small subunit of rat liver ribosomes were separated into five groups by stepwise elution from carboxymethylcellulose with LiCl at pH 6.5 (Collatz, E., Lin, A., Stöffler, G., Tsurugi, K., and Wool, I.G., (1976) J. Biol. Chem. 251, 1808-1816). From the several groups, 12 proteins (S2,S3, S4, S5, S6, S7, S8, S9, S13, S23/S24, S27, and S28) wereisolated by ion exchange chromatography on carboxymethylcellulose, by chromatography on sulfopropyl-Sephadex, and by gel filtration through Sephadex G-75. The amount of protein obtained varied from 1 to 9 mg depending on the number of steps required for the preparation; several proteins had no detectable contamination and the impurities in the others were no greater than 9%. The molecular weight of the proteins was estimated by polyazrylamide gel electrophoresis in sodium dodecyl sulfate; the amino acid composition was determined.
通过在pH 6.5条件下用LiCl从羧甲基纤维素上逐步洗脱，将大鼠肝脏核糖体小亚基的蛋白质分成了五组（科拉茨，E.，林，A.，施托弗勒，G.，鹤木，K.，以及伍尔，I.G.，（1976年）《生物化学杂志》251卷，1808 - 1816页）。从这几组中，通过在羧甲基纤维素上进行离子交换色谱、在磺丙基 - 葡聚糖凝胶上进行色谱以及通过葡聚糖凝胶G - 75进行凝胶过滤，分离出了12种蛋白质（S2、S3、S4、S5、S6、S7、S8、S9、S13、S23/S24、S27和S28）。根据制备所需的步骤数量，获得的蛋白质量在1至9毫克之间变化；几种蛋白质没有可检测到的污染物，其他蛋白质中的杂质不超过9%。通过在十二烷基硫酸钠中进行聚丙烯酰胺凝胶电泳估计蛋白质的分子量；测定氨基酸组成。
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