Department of Oral and Maxillofacial Surgery, University of Erlangen-Nürnberg, Glueckstraße 11, 91054, Erlangen, Germany.
Department of Oral and Maxillofacial Surgery, Medical University of Vienna, Vienna, Austria.
Clin Oral Investig. 2020 May;24(5):1651-1661. doi: 10.1007/s00784-019-03020-w. Epub 2019 Aug 15.
The preclinical study aimed to establish a standardized preclinical model to investigate osseous graft consolidation in defect configurations of limited regenerative capacity.
Critical size defects (CSD) were prepared and titanium tubes inserted for defect separation from local bone in the forehead area of 18 pigs. Defects were filled with demineralized bovine bone mineral (DBBM) or served as empty controls and were covered with a resorbable collagen membrane (CM) or left untreated. Six randomly selected pigs were sacrificed after 4, 8 and 12 weeks. Specimens were histologically and histomorphometrically analysed focusing on newly formed bone (NFB), demineralized bovine bone mineral (DBBM) and soft tissue (ST) proportions.
Four weeks after defect preparation, no statistically significant difference concerning NFB quantity could be detected within the groups. Defects covered with the CM showed lower amounts of DBBM. After 6 and 12 weeks, defects augmented with DBBM in combination with a CM (8 weeks: 43.12 ± 4.31; 12 weeks: 43.05 ± 3.01) showed a statistically significant higher NFB rate compared to empty control defects covered with 8 weeks: 7.66 ± 0.59; 12 weeks or without a CM; 8 weeks: 8.62 ± 2.66; 12 weeks: 18.40 ± 2.40. CM application showed no significant impact on osseous defect regeneration or soft tissue formation. Superior NFB could be detected for basal aspect for several evaluation time points.
The modification of CSD with titanium tubes represents a suitable model to imitate a one-wall defect regeneration situation.
The established model represents a promising method to evaluate graft consolidation in one-wall defect configuration.
本临床前研究旨在建立标准化的临床前模型，以研究在具有有限再生能力的缺陷构型中骨质移植物的整合。
在前额区域的 18 头猪中制备临界尺寸缺陷（CSD）并插入钛管，以使缺陷与局部骨分离。用脱矿牛骨矿物质（DBBM）填充缺陷或作为空对照组，并覆盖可吸收胶原膜（CM）或不做处理。6 只随机选择的猪在 4、8 和 12 周后被处死。标本进行组织学和组织形态计量学分析，重点关注新形成的骨（NFB）、脱矿牛骨矿物质（DBBM）和软组织（ST）的比例。
在缺陷制备后 4 周，各组之间 NFB 数量没有统计学差异。CM 覆盖的缺陷中 DBBM 含量较低。6 和 12 周后，与未覆盖 CM 的空对照组相比，用 DBBM 与 CM 联合填充的缺陷（8 周：43.12±4.31；12 周：43.05±3.01）显示出更高的 NFB 率，而未覆盖 8 周的空对照组；12 周或未覆盖 CM 的空对照组；8 周：7.66±0.59；12 周：18.40±2.40。CM 的应用对骨缺陷再生或软组织形成没有显著影响。在几个评估时间点都可以检测到基底方面的 NFB 更高。
用钛管对 CSD 的修改代表了一种模仿单壁缺陷再生情况的合适模型。
建立的模型代表了评估单壁缺陷构型中移植物整合的一种很有前途的方法。
