Rogova Varvara-Velika, Peev Stefan, Yotsova Ralitsa, Gerova-Vatsova Tsvetalina, Parushev Ivaylo
Department of Oral Surgery, Faculty of Dental Medicine, Medical University of Varna, 9002 Varna, Bulgaria.
Department of Periodontology and Dental Implantology, Faculty of Dental Medicine, Medical University of Varna, 9002 Varna, Bulgaria.
Materials (Basel). 2024 Dec 30;18(1):119. doi: 10.3390/ma18010119.
With the implementation of bone substitute materials, regeneration strategies have inevitably evolved over the years. Histomorphometry is the optimal means of quantitative evaluation of bone structure and morphology. This systematic review focuses on determining study models, staining methods and histomorphometric parameters used for bone regeneration research on non-decalcified plastic-embedded specimens over the last 10 years. After being subjected to the inclusion and exclusion criteria, 118 studies were included in this review. The results establish the most commonly selected animal model is rat, followed by rabbit, sheep and dog. Strong preference for staining samples with toluidine blue was noted. With regard to histomorphometric parameters, terms related to bone were most frequently assessed, amounting to almost half of recorded parameters. New bone formation was the main descriptor of this category. Residual bone graft and non-bone tissue parameters were also often evaluated. With regard to dynamic histomorphometry, mineral apposition rate (MAR) was the parameter of choice for most researchers, with calcein green being the preferred dye for fluorochrome labelling. An overview of the contemporary literature, as well as weaknesses in the current research protocols have been discussed.
随着骨替代材料的应用，多年来再生策略不可避免地发生了演变。组织形态计量学是定量评估骨结构和形态的最佳方法。本系统评价聚焦于确定过去10年用于非脱钙塑料包埋标本骨再生研究的研究模型、染色方法和组织形态计量学参数。经过纳入和排除标准筛选，本评价纳入了118项研究。结果表明，最常选用的动物模型是大鼠，其次是兔、绵羊和犬。发现对用甲苯胺蓝染色样本有强烈偏好。关于组织形态计量学参数，与骨相关的术语评估最为频繁，几乎占记录参数的一半。新骨形成是该类别的主要描述指标。残余骨移植和非骨组织参数也经常被评估。关于动态组织形态计量学，矿物质沉积率（MAR）是大多数研究人员选择的参数，钙黄绿素是荧光染料标记的首选染料。本文还讨论了当代文献综述以及当前研究方案中的不足。
